A Single-Component Luminescent Biosensor for the SARS-CoV-2 Spike Protein.
J Am Chem Soc
; 144(30): 13663-13672, 2022 08 03.
Article
in English
| MEDLINE | ID: covidwho-1960253
ABSTRACT
Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of Escherichia coli and Saccharomyces cerevisiae. This biosensor is deployed in multiple homogeneous and immobilized assay formats to detect recombinant SARS-CoV-2 spike antigen and cultured virus. The chemiluminescent signal generated facilitates detection by an unaugmented cell phone camera. Binding-activated tandem split-enzyme (BAT) biosensors may serve as a useful template for diagnostics and reagents that detect SARS-CoV-2 antigens and other proteins of interest.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Biosensing Techniques
/
COVID-19
Limits:
Humans
Language:
English
Journal:
J Am Chem Soc
Year:
2022
Document Type:
Article
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