Your browser doesn't support javascript.
A Single-Component Luminescent Biosensor for the SARS-CoV-2 Spike Protein.
Ravalin, Matthew; Roh, Heegwang; Suryawanshi, Rahul; Kumar, G Renuka; Pak, John E; Ott, Melanie; Ting, Alice Y.
  • Suryawanshi R; Gladstone Institutes, San Francisco, California 94158, United States.
  • Kumar GR; Gladstone Institutes, San Francisco, California 94158, United States.
  • Pak JE; Chan Zuckerberg Biohub, San Francisco, California 94158, United States.
  • Ott M; Gladstone Institutes, San Francisco, California 94158, United States.
  • Ting AY; Chan Zuckerberg Biohub, San Francisco, California 94158, United States.
J Am Chem Soc ; 144(30): 13663-13672, 2022 08 03.
Article in English | MEDLINE | ID: covidwho-1960253
ABSTRACT
Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of Escherichia coli and Saccharomyces cerevisiae. This biosensor is deployed in multiple homogeneous and immobilized assay formats to detect recombinant SARS-CoV-2 spike antigen and cultured virus. The chemiluminescent signal generated facilitates detection by an unaugmented cell phone camera. Binding-activated tandem split-enzyme (BAT) biosensors may serve as a useful template for diagnostics and reagents that detect SARS-CoV-2 antigens and other proteins of interest.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Limits: Humans Language: English Journal: J Am Chem Soc Year: 2022 Document Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Limits: Humans Language: English Journal: J Am Chem Soc Year: 2022 Document Type: Article