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Establishment of a Duplex Real Time PCR Method for Detection of SARS-COV-2
Integrated Ferroelectrics ; 228(1):183-191, 2022.
Article in English | ProQuest Central | ID: covidwho-1960724
ABSTRACT
To quickly and efficiently detect the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and prevent and control the spread of novel coronavirus disease (COVID-19), a highly sensitive duplex real-time PCR (RT-PCR) detection method has been established. In this study, the specificity of primers and probes were designed, respectively, according to the ORF1ab gene and N gene sequence of SARS-COV-2, and fluorescent probes were labeled with carboxyl fluorescein (FAM) and green fluorescent protein (VIC). The duplex RT-PCR method for detecting SARS-COV-2 with TaqMan probe was established, which has a limit of detection of 10 copies/µL, and the linear detection range of ORF1ab and N gene were 1.0 × 101-1.0 × 105 copies/µL and 1.0 × 101-1.0 × 106 copies/µL, respectively, realizing the simultaneous detection of ORF1ab and N genes in simulated SARS-COV-2 samples. The method has high sensitivity, accurate quantification, simple operation, and cost-saving, which can be used for rapid and efficient quantitative detection of SARS-COV-2.
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Full text: Available Collection: Databases of international organizations Database: ProQuest Central Type of study: Diagnostic study Language: English Journal: Integrated Ferroelectrics Year: 2022 Document Type: Article

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Full text: Available Collection: Databases of international organizations Database: ProQuest Central Type of study: Diagnostic study Language: English Journal: Integrated Ferroelectrics Year: 2022 Document Type: Article