Elucidating the neuropathophysiology of COVID-19 using quantum dot biomimetics of SARS-CoV-2
Colloidal Nanoparticles for Biomedical Applications XVII 2022
; 11977, 2022.
Article
in English
| Scopus | ID: covidwho-1962038
ABSTRACT
Quantum dots were encapsulated in polymeric phospholipid micelles conjugated to multiple ligands of SARS-CoV-2 spike protein to form fluorescent biomimetic nanoparticles for SARS-CoV-2 (COVID-QDs). Phosphatidylethanolaminepolyethylene glycol (PEPEG) was appended with bis(4-methylphenyl)sulfone to form PEPEGbis-sulfone and self-assembled into micelles around CdSe/CdS core/shell quantum dots via thin-film rehydration. The introduction of the bis-sulfone group the surface of the micelle-encapsulated quantum dots provides multiple sites for conjugation to his-tagged SARS-CoV-2 spike protein via a bisalkylation mechanism. Based on the eluted unconjugated fraction, we estimate that an average of seven spike proteins are conjugated per COVID-QD. We treated an in-vitro model system for the neurovascular unit (NVU) with these COVID-QD constructs to investigate the COVID-QDs, and by proxy SARS-CoV-2, may modulate the NVU leading to the COVID-19 associated neuropathophysiology. © 2022 SPIE
Biomimetic Nanoparticles; blood-brain barrier; Neuro-COVID; Neuroimmunology; Quantum Dots; SARS-CoV-2; Biomimetics; Cadmium compounds; Functional polymers; II-VI semiconductors; Liposomes; Medical applications; Micelles; Nanocrystals; Phospholipids; Proteins; Semiconductor quantum dots; 4-methylphenyl; CdSe/CdS (core-shell) quantum dots; Multiple ligands; Phospholipids micelles; Quantum dot; Spike protein; Coronavirus
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Databases of international organizations
Database:
Scopus
Language:
English
Journal:
Colloidal Nanoparticles for Biomedical Applications XVII 2022
Year:
2022
Document Type:
Article
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