Glycoinformatics approach for identifying target positions to inhibit initial binding of SARS-CoV-2 S1 protein to the host cell
Journal of Applied Biological Sciences
; 16(1):89-101, 2022.
Article
in English
| CAB Abstracts | ID: covidwho-1964344
ABSTRACT
COVID-19 outbreak is still threatening the public health. Therefore, in the middle of the pandemic, all kind of knowledge on SARS-CoV-2 may help us to find the solution. Determining the 3D structures of the proteins involved in host-pathogen interactions are of great importance in the fight against infection. Besides, post-translational modifications of the protein on 3D structure should be revealed in order to understand the protein function since these modifications are responsible for the host-pathogen interaction. Based on these, we predicted O-glycosylation and phosphorylation positions using full amino acid sequence of S1 protein. Candidate positions were further analyzed with enzyme binding activity, solvent accessibility, surface area parameters and the positions determined with high accuracy rate were used to design 3D O-glycoprotein structure of the S1 protein using carbohydrate force field. In addition, the interaction between the C-type lectin CD209L and a-mannose residues was examined and carbohydrate recognition positions were predicted. We suggest these positions as a potential target for the inhibition of the initial binding of SARS-CoV-2 S1 protein to the host cell.
Prion, Viral, Bacterial and Fungal Pathogens of Humans [VV210]; Animal Models of Human Diseases [VV400]; Molecular Biology and Molecular Genetics [ZZ360]; amino acid sequences; binding; coronavirus disease 2019; inhibition; lectins; mannose; outbreaks; pandemics; phosphorylation; proteins; public health; viral diseases; viral proteins; Severe acute respiratory syndrome coronavirus 2; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; protein sequences; SARS-CoV-2; viral infections
Full text:
Available
Collection:
Databases of international organizations
Database:
CAB Abstracts
Language:
English
Journal:
Journal of Applied Biological Sciences
Year:
2022
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS