PRESERVED SARS-COV-2 VACCINE CELL-MEDIATED IMMUNOGENICITY IN INFLAMMATORY BOWEL DISEASE PATIENTS ON IMMUNEMODULATING THERAPIES
Gastroenterology
; 162(7):S-1008, 2022.
Article
in English
| EMBASE | ID: covidwho-1967396
ABSTRACT
BACKGROUND:
Immune-modulating medications for inflammatory bowel diseases (IBD) have been associated with suboptimal vaccine responses. There is conflicting data with SARS-CoV-2 vaccination.METHODS:
We measured SARS-CoV-2 vaccine immunogenicity at 2 weeks post 2nd mRNA vaccine in IBD patients as compared to normal healthy donors (NHD). We measured humoral immune responses to SARS-CoV-2 anti-spike Immunoglobulin G (IgG) and anti-receptor binding domain (RBD) IgG were measured by ELISA, and neutralizing antibody titers were measured using recombinant, reporter SARS-CoV-2. Antigen specific memory B cells were measured using recombinant SARS-CoV-2 proteins. Activation induced marker T cell (AIM) assays were performed using SARS-CoV-2 spike megapools. Immunophenotyping was performed by flow cytometry.RESULTS:
We enrolled 29 patients with IBD (19 with Crohn's disease, 10 with ulcerative colitis) on infliximab (IFX) monotherapy (N=9), IFX combination therapy with a thiopurine (N=9), vedolizumab monotherapy (N= 11) as compared to matched NHD (N=12). At 2 weeks post vaccination, all subjects made detectable anti-spike IgG and anti-RBD IgG. There were no differences in anti-spike IgG titers among the different groups. IBD patients on IFX monotherapy, but not IBD patients on IFX combination therapy or vedolizumab monotherapy, had lower anti-RBD and neutralization titers as compared to NHD (p-value 0.041 and 0.023, respectively) (Fig. 1). There were no significant differences in the percentage of spike-specific or RBD-specific memory B cells in IBD patients as compared to NHD (Fig. 1). There were no differences in the percentage of spike-specific CD4+ or CD8+ T cells in all IBD patients as compared to NHDs (Fig. 2).CONCLUSIONS:
We demonstrate overall comparable and perserved cell-mediated immunity to SARS-CoV-2 vaccination in a small cohort of IBD patients treated with a range of different immune-modulating medications as compared to healthy controls. Larger numbers of patients are needed to validate these findings.
CD4 antigen; endogenous compound; immunoglobulin G; infliximab; neutralizing antibody; RNA vaccine; SARS-CoV-2 vaccine; vedolizumab; adult; CD8+ T lymphocyte; cell assay; cellular immunity; clinical article; cohort analysis; conference abstract; controlled study; Crohn disease; drug combination; drug therapy; enzyme linked immunosorbent assay; female; flow cytometry; human; human cell; humoral immunity; immunophenotyping; inflammatory bowel disease; male; memory B lymphocyte; monotherapy; nonhuman; receptor binding; Severe acute respiratory syndrome coronavirus 2; spike; T lymphocyte; ulcerative colitis; vaccination; vaccine immunogenicity
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Topics:
Vaccines
Language:
English
Journal:
Gastroenterology
Year:
2022
Document Type:
Article
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