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Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs.
Wang, Busen; Xu, Jinghan; Wu, Shipo; Zhang, Zhe; Zhao, Zhenghao; Zhang, Jun; Fu, Ling; Zai, Xiaodong; Wang, Yudong; Zhang, Guanying; Chen, ZhengShan; Chen, Yi; Sun, Hancong; Song, Xiaohong; Zhang, Jinlong; Zhu, Lianhui; Hou, Lihua; Chen, Wei.
  • Wang B; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Xu J; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Wu S; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Zhang Z; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Zhao Z; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Zhang J; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Fu L; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Zai X; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Wang Y; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Zhang G; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Chen Z; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Chen Y; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Sun H; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Song X; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Zhang J; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Zhu L; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China.
  • Hou L; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China. houlihua@sina.com.
  • Chen W; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, 100071, Beijing, China. cw0226@foxmail.com.
Signal Transduct Target Ther ; 7(1): 257, 2022 Jul 29.
Article in English | MEDLINE | ID: covidwho-1967591
ABSTRACT
Highly divergent SARS-CoV-2 variants have continuously emerged and spread around the world, and updated vaccines and innovative vaccination strategies are urgently needed to address the global SARS-COV2 pandemic. Here, we established a series of Ad5-vectored SARS-CoV-2 variant vaccines encoding multiple spike proteins derived from the Alpha, Beta, Gamma, Epsilon, Kappa, Delta and Omicron lineages and analyzed the antibody immune responses induced by single-dose and prime-boost vaccination strategies against emerging SARS-CoV-2 variants of concern (VOCs). Single-dose vaccination with SARS-CoV-2 variant vaccines tended to elicit the optimal self-matched neutralizing effects, and Ad5-B.1.351 produced more broad-spectrum cross-neutralizing antibodies against diverse variants. In contrast, prime-boost vaccination further strengthened and broadened the neutralizing antibody responses against highly divergent SARS-CoV-2 variants. The heterologous administration of Ad5-B.1.617.2 and Ad5-B.1.429 to Ad5-WT-primed mice resulted in superior antibody responses against most VOCs. In particular, the Omicron spike could only stimulate self-matched neutralizing antibodies with infrequent cross-reactivities to other variants used in single-dose vaccination strategies; moreover, with prime-boost regimens, this vaccine elicited an optimal specific neutralizing antibody response to Omicron, and prompted cross-antibody responses against other VOCs that were very similar to those obtained with Ad5-WT booster. Overall, this study delineated the unique characteristics of antibody responses to the SARS-CoV-2 VOC spikes with the single-dose or prime-boost vaccination strategies and provided insight into the vaccine development of next SARS-CoV-2 VOCs.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / COVID-19 Type of study: Randomized controlled trials Topics: Vaccines / Variants Limits: Animals / Humans Language: English Journal: Signal Transduct Target Ther Year: 2022 Document Type: Article Affiliation country: S41392-022-01065-0

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / COVID-19 Type of study: Randomized controlled trials Topics: Vaccines / Variants Limits: Animals / Humans Language: English Journal: Signal Transduct Target Ther Year: 2022 Document Type: Article Affiliation country: S41392-022-01065-0