Significance of mucosal associated invariant T (MAIT) Cells in SARS-CoV-2 infection
Scandinavian Journal of Immunology
; 95(6), 2022.
Article
in English
| EMBASE | ID: covidwho-1968186
ABSTRACT
In the COVID-19 disease caused by SARS-CoV-2 virus prolonged T cell lymphopenia is common but the mechanism for it is still unknown. More importantly, the T cell lymphopenia is associated with more severe diseases. The lymphopenia in COVID-19 is more pronounced in a specialized innate-like T cell population called Mucosal Associated Invariant T cells (MAITs). MAITs are the only T cell population that expresses ACE2 receptor that SARS-CoV-2 uses for cell entry. This project aims to decipher the pathogenetic process leading to MAITs depletion in COVID-19. We hypothesize that SARS-CoV-2 can directly activate MAITs which leads to MAITs overactivation and excessive tissue destruction in the lungs where MAITs comprise a large part of the tissue-resident T cells. To test these hypotheses, we challenged MAITs isolated from healthy blood donors and controls from different age groups with SARS-CoV-2 in vitro in a BSL3 laboratory. We used a combination of multi-colour flow cytometry, RT-PCR and in situ lung immunohistochemistry to evaluate the changes in MAIT cells' functions and activation. We then translated the in vitro findings to in vivo and measured similar MAIT cell activation signatures from actual real-life patient samples obtained during acute COVID-19. Our study shows that human circulation MAIT cells are activated and declined in patients with SARS-CoV-2 infection. SARS-CoV-2 infects MAIT cells in a non-direct way.
angiotensin converting enzyme 2; endogenous compound; adult; biosafety level 3; blood donor; cell function; cell population; conference abstract; controlled study; coronavirus disease 2019; destruction; flow cytometry; groups by age; histopathology; human; human cell; human tissue; immunohistochemistry; in vitro study; in vivo study; lung; mucosal-associated invariant T cell; nonhuman; protein expression; resident; Severe acute respiratory syndrome coronavirus 2; T lymphocyte; T lymphocyte activation
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
Scandinavian Journal of Immunology
Year:
2022
Document Type:
Article
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