Electroporation: An Effective Method For In Vivo Gene Delivery
Drug Delivery Letters
; 12(1):35-45, 2022.
Article
in English
| EMBASE | ID: covidwho-1968951
ABSTRACT
Background:
Gene therapy is a promising approach for the treatment of various diseases, including cancer, hereditary disorders, and some viral infections. The development of efficient and safe gene delivery systems is essential for facilitating gene transfer to various organs and tissues in vivo.Objective:
In this review, we briefly describe the principal mechanisms of gene delivery systems, particularly electroporation, and discuss the latest advancements in the application of electro-poration for in vivo gene transfer.Methods:
A narrative review of all the relevant publication known to the authors was conducted.Results:
In recent years, electroporation-based strategies have emerged as an auspicious and versa-tile platform for efficient and controlled delivery of various biomolecules, including nucleic acids. Applying electric pulses of enough magnitude leads to the formation of hydrophilic pores in the cell membrane and allows the entry of otherwise membrane-impermeant molecules, such as DNA. Alt-hough electroporation has been initially developed for in vitro transfection of cells, it has recently advanced to preclinical in vivo applications and finally to clinical trials.Conclusion:
Electroporation has already entered the clinical practice for antitumor therapy and may be an essential part of future personalized treatments. Given the ability of electroporation to deliver multiple genes in a single event, it will also certainly be further developed both as a stand-alone delivery approach and when coupled with other technologies.
NCT02204098; NCT02772003; NCT03122106; NCT03199040; NCT03439085; NCT03491683; NCT03532217; NCT03603808; NCT03718130; NCT04131413; NCT04251117; NCT04336410; NCT04397003; NCT04627675; NCT04642638; NCT04736147; NCT04788459; NCT04906629; amino acid; arginine; lysine; Adeno associated virus; cancer therapy; coronavirus disease 2019; electroporation; enzymatic degradation; gene delivery system; gene expression; gene therapy; gene transfer; genetic disorder; human; immune response; in vivo gene transfer; malignant neoplasm; microinjection; nonhuman; Retroviridae; review; SARS coronavirus; ultrasound; urogenital system; viral gene delivery system; virus infection
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Type of study:
Experimental Studies
Language:
English
Journal:
Drug Delivery Letters
Year:
2022
Document Type:
Article
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