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Evolution of the PB1 gene of human influenza A (H3N2) viruses circulating between 1968 and 2019.
Sun, Tongtong; Guo, Yanna; Zhao, Lingcai; Fan, Menglu; Huang, Nan; Tian, Miao; Liu, Qingzheng; Huang, Jingjin; Liu, Zhiyuan; Zhao, Yongzhen; Ji, Zhiwei; Ping, Jihui.
  • Sun T; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • Guo Y; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • Zhao L; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • Fan M; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • Huang N; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • Tian M; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • Liu Q; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • Huang J; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • Liu Z; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • Zhao Y; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • Ji Z; College of Artificial Intelligence, Nanjing Agricultural University, Nanjing, China.
  • Ping J; MOE International Joint Collaborative Research Laboratory for Animal Health and Food Safety & Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Transbound Emerg Dis ; 69(4): 1824-1836, 2022 Jul.
Article in English | MEDLINE | ID: covidwho-1973738
Semantic information from SemMedBD (by NLM)
1. PBRM1 gene|PBRM1 PART_OF Virus
Subject
PBRM1 gene|PBRM1
Predicate
PART_OF
Object
Virus
2. PBRM1 gene|PBRM1 PART_OF Virus
Subject
PBRM1 gene|PBRM1
Predicate
PART_OF
Object
Virus
ABSTRACT
One avian H3N2 influenza virus, providing its PB1 and HA segments, reassorted with one human H2N2 virus and caused a pandemic outbreak in 1968, killing over 1 million people. After its introduction to humanity, the pandemic H3N2 virus continued adapting to humans and has resulted in epidemic outbreaks every influenza season. To understand the functional roles of the originally avian PB1 gene in the circulating strains of human H3N2 influenza viruses, we analyzed the evolution of the PB1 gene in all human H3N2 isolates from 1968 to 2019. We found several specific residues dramatically changed around 2002-2009 and remained stable through to 2019. Then, we verified the functions of these PB1 mutations in the genetic background of the early pandemic virus, A/Hong Kong/1/1968(HK/68), as well as a recent seasonal strain, A/Jiangsu/34/2016 (JS/16). The PB1 V709I or PB1 V113A/K586R/D619N/V709I induced higher polymerase activity of HK/68 in human cells. And the four mutations acted cooperatively that had an increased replication capacity in vitro and in vivo at an early stage of infection. In contrast, the backward mutant, A113V/R586K/N619D/I709V, reduced polymerase activity in human cells. The PB1 I709V decreased viral replication in vitro, but this mutant only showed less effect on mice infection experiment, which suggested influenza A virus evolved in human host was not always consisted with highly replication efficiency and pathogenicity in other mammalian host. Overall, our results demonstrated that the identified PB1 mutations contributed to the viral evolution of human influenza A (H3N2) viruses.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Influenza A virus / Rodent Diseases / Influenza, Human / Influenza in Birds Type of study: Observational study Limits: Animals / Humans Language: English Journal: Transbound Emerg Dis Journal subject: Veterinary Medicine Year: 2022 Document Type: Article Affiliation country: Tbed.14161

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Influenza A virus / Rodent Diseases / Influenza, Human / Influenza in Birds Type of study: Observational study Limits: Animals / Humans Language: English Journal: Transbound Emerg Dis Journal subject: Veterinary Medicine Year: 2022 Document Type: Article Affiliation country: Tbed.14161