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Characterization and cross-protection of experimental infections with SeCoV and two PEDV variants.
Puente, Héctor; Díaz, Ivan; Arguello, Héctor; Mencía-Ares, Óscar; Gómez-García, Manuel; Pérez-Pérez, Lucía; Vega, Clara; Cortey, Martí; Martín, Margarita; Rubio, Pedro; Carvajal, Ana.
  • Puente H; Facultad de Veterinaria, Departamento de Sanidad Animal, Universidad de León, León, Spain.
  • Díaz I; IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Universitat Autònoma de Barcelona, Bellaterra, Spain.
  • Arguello H; Facultad de Veterinaria, Departamento de Sanidad Animal, Universidad de León, León, Spain.
  • Mencía-Ares Ó; INDEGSAL, Instituto de Desarrollo Ganadero, Universidad de León, León, Spain.
  • Gómez-García M; Facultad de Veterinaria, Departamento de Sanidad Animal, Universidad de León, León, Spain.
  • Pérez-Pérez L; Facultad de Veterinaria, Departamento de Sanidad Animal, Universidad de León, León, Spain.
  • Vega C; Facultad de Veterinaria, Departamento de Sanidad Animal, Universidad de León, León, Spain.
  • Cortey M; Facultad de Veterinaria, Departamento de Sanidad Animal, Universidad de León, León, Spain.
  • Martín M; Facultat de Veterinària, Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain.
  • Rubio P; Facultat de Veterinària, Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain.
  • Carvajal A; Facultad de Veterinaria, Departamento de Sanidad Animal, Universidad de León, León, Spain.
Transbound Emerg Dis ; 2022 Aug 02.
Article in English | MEDLINE | ID: covidwho-1973746
ABSTRACT
The aim of this study was to characterize the infection of weaned pigs with swine enteric coronavirus (SeCoV) - a chimeric virus most likely originated from a recombination event between porcine epidemic diarrhoea virus (PEDV) and transmissible gastroenteritis virus, or its mutant porcine respiratory coronavirus - and two PEDV G1b variants, including a recently described recombinant PEDV-SeCoV (rPEDV-SeCoV), as well as to determine the degree of cross-protection achieved against the rPEDV-SeCoV. For this purpose, forty-eight 4-week-old weaned pigs were randomly allocated into four groups of 12 animals. Piglets within each group were primary inoculated with one of the investigated viral strains (B PEDV; C SeCoV and D rPEDV-SeCoV) or mock-inoculated (A), and exposed to rPEDV-SeCOV at day 20 post-infection; thus, group A was primary challenged (-/rPEDV-SeCoV), groups B and C were subjected to a heterologous re-challenge (PEDV/rPEDV-SeCoV and SeCoV/rPEDV-SeCoV, respectively), and group D to a homologous re-challenge (rPEDV-SeCoV/rPEDV-SeCoV), Clinical signs, viral shedding, microscopic lesions and specific humoral and cellular immune responses (IgG, IgA, neutralizing antibodies and IgA and IFN-γ-secreting cells) were monitored. After primo-infection, all three viral strains induced an undistinguishable mild-to-moderate clinical disease with diarrhoea as the main sign and villus shortening lesions in the small intestine. In homologous re-challenged pigs, no clinical signs or lesions were observed, and viral shedding was only detected in a single animal. This fact may be explained by the significant high level of rPEDV-SeCoV-specific neutralizing antibodies found in these pigs before the challenge. In contrast, prior exposure to a different PEDV G1b variant or SeCoV only provided partial cross-protection, allowing rPEDV-SeCoV replication and shedding in faeces.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Topics: Variants Language: English Journal subject: Veterinary Medicine Year: 2022 Document Type: Article Affiliation country: Tbed.14674

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Topics: Variants Language: English Journal subject: Veterinary Medicine Year: 2022 Document Type: Article Affiliation country: Tbed.14674