Your browser doesn't support javascript.
Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunity.
López-Muñoz, Alberto Domingo; Kosik, Ivan; Holly, Jaroslav; Yewdell, Jonathan W.
  • López-Muñoz AD; Cellular Biology Section, Laboratory of Viral Diseases, NIAID (NIH), Bethesda, MD, USA.
  • Kosik I; Cellular Biology Section, Laboratory of Viral Diseases, NIAID (NIH), Bethesda, MD, USA.
  • Holly J; Cellular Biology Section, Laboratory of Viral Diseases, NIAID (NIH), Bethesda, MD, USA.
  • Yewdell JW; Cellular Biology Section, Laboratory of Viral Diseases, NIAID (NIH), Bethesda, MD, USA.
Sci Adv ; 8(31): eabp9770, 2022 08 05.
Article in English | MEDLINE | ID: covidwho-1973775
ABSTRACT
SARS-CoV-2 nucleocapsid protein (N) induces strong antibody (Ab) and T cell responses. Although considered to be localized in the cytosol, we readily detect N on the surface of live cells. N released by SARS-CoV-2-infected cells or N-expressing transfected cells binds to neighboring cells by electrostatic high-affinity binding to heparan sulfate and heparin, but not other sulfated glycosaminoglycans. N binds with high affinity to 11 human chemokines, including CXCL12ß, whose chemotaxis of leukocytes is inhibited by N from SARS-CoV-2, SARS-CoV-1, and MERS-CoV. Anti-N Abs bound to the surface of N-expressing cells activate Fc receptor-expressing cells. Our findings indicate that cell surface N manipulates innate immunity by sequestering chemokines and can be targeted by Fc-expressing innate immune cells. This, in combination with its conserved antigenicity among human CoVs, advances its candidacy for vaccines that induce cross-reactive B and T cell immunity to SARS-CoV-2 variants and other human CoVs, including novel zoonotic strains.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Middle East Respiratory Syndrome Coronavirus / COVID-19 Type of study: Randomized controlled trials Topics: Vaccines / Variants Limits: Humans Language: English Journal: Sci Adv Year: 2022 Document Type: Article Affiliation country: Sciadv.abp9770

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Middle East Respiratory Syndrome Coronavirus / COVID-19 Type of study: Randomized controlled trials Topics: Vaccines / Variants Limits: Humans Language: English Journal: Sci Adv Year: 2022 Document Type: Article Affiliation country: Sciadv.abp9770