Dynamic changes in production of antibodies against SARS-CoV-2 based on three serological kits
Chinese Journal of Virology
; 36(3):343-347, 2020.
Article
in Chinese
| GIM | ID: covidwho-1975407
ABSTRACT
We wished to understand the dynamic changes in production of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies in sera collected from coronavirus disease 19 (COVID-19) patients. Fifty-eight serum samples from 33 patients confirmed to have COVID-19 in Gansu Province, China, were tested for three types of SARS-CoV-2-specific antibodies immunoglobulin (Ig) M, IgG, and total antibodies. The positive rate of IgM, IgG and total antibodies increased gradually with COVID-19 progression. Within the first 3 days, the positive rate of detection of SARS-CoV-2-specific antibody using the three kits was 13.6%-31.8%. whereas, within 4-7 days, it was 36.4%-45.5%, within 8-14 days it was 55.6%-77.8%, and after 15 days, it was 100%. In addition, the three kits were used to measure antibodies from serum samples collected from healthy people, and the specificity was 99%-100%. Statistical analyses indicated no significant difference among the results of the three kits (P > 0.05 for all). In summary, the three SARS-CoV-2 antibody-detection kits had good sensitivity and specificity for detection of antibodies against SARS-CoV-2, and could aid the clinical diagnosis of COVID-19. The dynamic characteristics of production of SARS-CoV-2- specific antibodies could provide important scientific bases for epidemiologic investigations.
308067-58-5; antibodies; antibody formation; antibody testing; blood serum; coronavirus disease 2019; diagnosis; disease course; human diseases; IgG; IgM; immunoassay; immunological techniques; pandemics; serology; viral diseases; man; Severe acute respiratory syndrome coronavirus 2; China; Gansu; APEC countries; East Asia; Asia; high Human Development Index countries; upper-middle income countries; North Western China; Homo; Hominidae; primates; mammals; vertebrates; Chordata; animals; eukaryotes; Severe acute respiratory syndrome-related coronavirus; Betacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; antibody detection; antibody tests; People's Republic of China; disease progression; Kansu; serological techniques; SARS-CoV-2; viral infections
Full text:
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Collection:
Databases of international organizations
Database:
GIM
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2020
Document Type:
Article
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