Immunogenicity of genetic vaccine based on recombinant hepatitis B Core antigen and B cell multi-epitopes of Porcine epidemic diarrhea virus spike glycoprotein
Chinese Veterinary Science / Zhongguo Shouyi Kexue
; 50(6):671-678, 2020.
Article
in Chinese
| CAB Abstracts | ID: covidwho-1975502
ABSTRACT
In present study, in order to develop a new and effective porcine epidemic diarrhea virus (PEDV)vaccine, three B cell epitopes and the truncated S1 gene of PEDV spike protein were combined and inserted into the immunodominant region of the HBcAg. Then the constructed recombinant plasmid HBcAg-PE was transformed to E. coli BL21 (DE3) for expression. After purification and identification by Western-blot, the expressed recombinant proteins HBPE were injected into BALB/c mice as vaccine antigen with different doses through intramuscular injection and its immune effect were preliminary evaluated. The results showed that the recombinant proteins HBPE was expressed as precipitation form and it could reacted specifically with PEDV-positive serum after purification and renaturation. Besides, the RH could induce anti-PEDV specific antibodies and the related Thl and Th2 cytokines in mice. The above results indicate that the recombinant compound epitope antigen of PEDV was successfully constructed. and its immunogenicity as a new vaccine candidate was evaluated in the mice in this study. The results of this study provided a new idea for the development of PEDV genetic engineering vaccine in the future.
Diagnostic, Therapeutic and Pharmacological Biotechnology [WW700]; Prion, Viral, Bacterial and Fungal Pathogens of Animals [LL821]; Animal Immunology [LL650]; Host Resistance and Immunity [HH600]; Genetic Engineering, Gene Transfer and Transgenics [WW100]; vaccine development; immunogenicity; DNA vaccines; recombinant proteins; disease prevention; disease control; laboratory animals; genetic engineering; mice; Porcine epidemic diarrhea virus; pigs; Muridae; rodents; mammals; vertebrates; Chordata; animals; eukaryotes; Alphacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; Sus scrofa; Sus; Suidae; Suiformes; Artiodactyla; recombinant plasmid; Porcine epidemic diarrhoea virus; swine; hogs; genetic manipulation
Full text:
Available
Collection:
Databases of international organizations
Database:
CAB Abstracts
Topics:
Vaccines
Language:
Chinese
Journal:
Zhongguo Shouyi Kexue
Year:
2020
Document Type:
Article
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