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Direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer.
Connelly, Guinevere G; Kirkland, Orville O; Bohannon, Seven; Lim, Daniel C; Wilson, Robert M; Richards, Edward J; Tay, Dousabel M; Jee, Hyuk; Hellinger, Riley D; Hoang, Ngoc K; Hao, Liang; Chhabra, Arnav; Martin-Alonso, Carmen; Tan, Edward K W; Koehler, Angela N; Yaffe, Michael B; London, Wendy B; Lee, Pui Y; Krammer, Florian; Bohannon, Robert C; Bhatia, Sangeeta N; Sikes, Hadley D; Li, Hojun.
  • Connelly GG; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Kirkland OO; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Bohannon S; Catalloid Products, Elkhart, IN 46514, USA.
  • Lim DC; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Wilson RM; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Richards EJ; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Tay DM; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Jee H; Dragonfly Therapeutics, Waltham, MA 02451, USA.
  • Hellinger RD; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Hoang NK; Division of Rheumatology, Boston Children's Hospital, Boston, MA 02115, USA.
  • Hao L; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Chhabra A; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Martin-Alonso C; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Tan EKW; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Koehler AN; Satellite Bio, Cambridge, MA 02139, USA.
  • Yaffe MB; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • London WB; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Lee PY; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Krammer F; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Bohannon RC; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Bhatia SN; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Sikes HD; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Li H; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Cell Rep Methods ; 2(8): 100273, 2022 Aug 22.
Article in English | MEDLINE | ID: covidwho-1977166
ABSTRACT
Neutralizing antibody (NAb) titer is a key biomarker of protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, but point-of-care methods for assessing NAb titer are not widely available. Here, we present a lateral flow assay that captures SARS-CoV-2 receptor-binding domain (RBD) that has been neutralized from binding angiotensin-converting enzyme 2 (ACE2). Quantification of neutralized RBD in this assay correlates with NAb titer from vaccinated and convalescent patients. This methodology demonstrated superior performance in assessing NAb titer compared with either measurement of total anti-spike immunoglobulin G titer or quantification of the absolute reduction in binding between ACE2 and RBD. Our testing platform has the potential for mass deployment to aid in determining at population scale the degree of protective immunity individuals may have following SARS-CoV-2 vaccination or infection and can enable simple at-home assessment of NAb titer.
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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Journal: Cell Rep Methods Year: 2022 Document Type: Article Affiliation country: J.CRMETH.2022.100273

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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Journal: Cell Rep Methods Year: 2022 Document Type: Article Affiliation country: J.CRMETH.2022.100273