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Electrochemical Biosensor for SARS-CoV-2 cDNA Detection Using AuPs-Modified 3D-Printed Graphene Electrodes.
Silva, Luiz R G; Stefano, Jéssica S; Orzari, Luiz O; Brazaca, Laís C; Carrilho, Emanuel; Marcolino-Junior, Luiz H; Bergamini, Marcio F; Munoz, Rodrigo A A; Janegitz, Bruno C.
  • Silva LRG; Department of Nature Sciences, Mathematics and Education, Federal University of São Carlos, Araras 13600-970, SP, Brazil.
  • Stefano JS; Department of Physics, Chemistry and Mathematics, Federal University of São Carlos, Sorocaba 18052-780, SP, Brazil.
  • Orzari LO; Department of Nature Sciences, Mathematics and Education, Federal University of São Carlos, Araras 13600-970, SP, Brazil.
  • Brazaca LC; Department of Nature Sciences, Mathematics and Education, Federal University of São Carlos, Araras 13600-970, SP, Brazil.
  • Carrilho E; Department of Physics, Chemistry and Mathematics, Federal University of São Carlos, Sorocaba 18052-780, SP, Brazil.
  • Marcolino-Junior LH; São Carlos Institute of Chemistry (IQSC), University of São Paulo (USP), São Carlos 13566-590, SP, Brazil.
  • Bergamini MF; National Institute of Science and Technology in Bioanalysis-INCTBio, Campinas 13083-970, SP, Brazil.
  • Munoz RAA; São Carlos Institute of Chemistry (IQSC), University of São Paulo (USP), São Carlos 13566-590, SP, Brazil.
  • Janegitz BC; National Institute of Science and Technology in Bioanalysis-INCTBio, Campinas 13083-970, SP, Brazil.
Biosensors (Basel) ; 12(8)2022 Aug 10.
Article in English | MEDLINE | ID: covidwho-1979122
ABSTRACT
A low-cost and disposable graphene polylactic (G-PLA) 3D-printed electrode modified with gold particles (AuPs) was explored to detect the cDNA of SARS-CoV-2 and creatinine, a potential biomarker for COVID-19. For that, a simple, non-enzymatic electrochemical sensor, based on a Au-modified G-PLA platform was applied. The AuPs deposited on the electrode were involved in a complexation reaction with creatinine, resulting in a decrease in the analytical response, and thus providing a fast and simple electroanalytical device. Physicochemical characterizations were performed by SEM, EIS, FTIR, and cyclic voltammetry. Square wave voltammetry was employed for the creatinine detection, and the sensor presented a linear response with a detection limit of 0.016 mmol L-1. Finally, a biosensor for the detection of SARS-CoV-2 was developed based on the immobilization of a capture sequence of the viral cDNA upon the Au-modified 3D-printed electrode. The concentration, immobilization time, and hybridization time were evaluated in presence of the DNA target, resulting in a biosensor with rapid and low-cost analysis, capable of sensing the cDNA of the virus with a good limit of detection (0.30 µmol L-1), and high sensitivity (0.583 µA µmol-1 L). Reproducible results were obtained (RSD = 1.14%, n = 3), attesting to the potentiality of 3D-printed platforms for the production of biosensors.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 / Graphite Type of study: Diagnostic study / Experimental Studies Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Bios12080622

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 / Graphite Type of study: Diagnostic study / Experimental Studies Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Bios12080622