Clostridium perfringens Spores in Urology Hospital
Jundishapur Journal of Microbiology
; 15(5), 2022.
Article
in English
| EMBASE | ID: covidwho-1979586
ABSTRACT
Background:
Clostridium spp. spores are resistant to many factors, including alcohol-based disinfectants. The presence of clostridial spores in a hospital environment may lead to infection outbreaks among patients and health care workers.Objective:
This study is aimed to detect clostridial spores in the aurology hospital using C diff Banana Broth™ and assess the antibiotic sensitivity and toxinotypes of isolates.Methods:
After diagnosing COVID-19 in medical staff and closing an 86-bed urology hospital in 2020 for H2O2 fogging, 58 swabs from the hospital environment were inoculated to C diff Banana Broth™, incubated at 37°C for 14 days, checked daily, and positive broths were sub-cultured anaerobically for 48 h at 37°C. After identification, multiplex PCR (mPCR) was performed for Clostridium perfringens, C. difficile toxin genes, and minimum inhibitory concentration (MIC) determination.Results:
In this study, 16 out of 58 (~ 28%) strains of Clostridium spp. were cultured 11-C. perfringens, 2-C. baratii, and 1 each of C. paraputrificum, C. difficile, and C. clostridioforme. 11 C. perfringens were positive for the cpa, 7-the cpb2, 2 – cpiA, and 1 – cpb toxin genes. All isolates were sensitive to metronidazole, vancomycin, moxifloxacin, penicillin/tazobactam, and rifampicin. Two out of the 11 C. perfringens strains were resistant to erythromycin and clindamycin.Conclusions:
Regardless of the performed H2O2 fogging, antibiotic-resistant, toxigenic strains of C. perfringens (69%) obtained from the urology hospital environment were cultured using C diff Banana Broth™, indicating the need to develop the necessary sanitary and epidemiological procedures in this hospital.
antibiotic resistance; antibiotic sensitivity; article; bacterial spore; bacterial strain; bacterium culture; bacterium isolate; banana; clinical article; Clostridioides difficile; Clostridium; Clostridium perfringens; controlled study; coronavirus disease 2019; human cell; medical staff; minimum inhibitory concentration; multiplex polymerase chain reaction; nonhuman; urology; clindamycin; erythromycin; hydrogen peroxide; metronidazole; moxifloxacin; penicillin derivative; rifampicin; tazobactam; toxin; vancomycin
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
Jundishapur Journal of Microbiology
Year:
2022
Document Type:
Article
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