KINETIC AND INHIBITION EFFECT STUDIES OF ECOFRIENDLY SYNTHESIZED GOLD NANOPARTICLES ON LACTATE DEHYDROGENASE AND FERRITIN ACTIVITY

ABSTRACT

This paper examines the biological approach used to synthesize gold nanoparticles (AuNPs) by using Myrtus communis leaves extract as reducing agent and Chloroauric acid as precursors. Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) used in addition to UV-visible spectroscopy (UV) in order to characterize the AuNPs. The biosynthesized AuNPs exhibited inhibitory effects on ferritin and lactate dehydrogenase activity in sera of covid-19 patients and the sera of healthy control subjects;the inhibition percentage with LDH was 76.86% and 63% for patient and control group, respectively. While for ferritin activity, it was 85.85% for patient group and 75.18% for control group. Kinetic studies of ferritin and lactate dehydrogenase performed. Further studies on other biological activities were required to exploit AuNPs full potential. The goal of this study is to synthesized gold nanoparticles using simple, cheap and environmentally green method. This stage is more suited to large-scale manufacturing since it is speedy and removes the complex steps in other bio based methods (by using fungi and bacteria).