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Anisodamine potently inhibits SARS-CoV-2 infection in vitro and targets its main protease.
Wei, Wei; Kong, Ni; Liu, Meng-Zhen; Han, Ting; Xu, Jun-Feng; Liu, Chong.
  • Wei W; Department of Pharmacy, Naval Medical University/Second Military Medical University, Shanghai, 200433, China; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sci
  • Kong N; Department of Pharmacy, Naval Medical University/Second Military Medical University, Shanghai, 200433, China.
  • Liu MZ; Department of Pharmacy, Naval Medical University/Second Military Medical University, Shanghai, 200433, China.
  • Han T; Department of Pharmacy, Naval Medical University/Second Military Medical University, Shanghai, 200433, China.
  • Xu JF; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
  • Liu C; Department of Pharmacy, Naval Medical University/Second Military Medical University, Shanghai, 200433, China. Electronic address: cliuflying@smmu.edu.cn.
Biochem Biophys Res Commun ; 616: 8-13, 2022 08 06.
Article in English | MEDLINE | ID: covidwho-1982607
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) provoked a pandemic of acute respiratory disease, namely coronavirus disease 2019 (COVID-19). Currently, effective drugs for this disease are urgently warranted. Anisodamine is a traditional Chinese medicine that is predicted as a potential therapeutic drug for the treatment of COVID-19. Therefore, this study aimed to investigate its antiviral activity and crucial targets in SARS-CoV-2 infection. SARS-CoV-2 and anisodamine were co-cultured in Vero E6 cells, and the antiviral activity of anisodamine was assessed by immunofluorescence assay. The antiviral activity of anisodamine was further measured by pseudovirus entry assay in HEK293/hACE2 cells. Finally, the predictions of crucial targets of anisodamine on SARS-CoV-2 were analyzed by molecular docking studies. We discovered that anisodamine suppressed SARS-CoV-2 infection in Vero E6 cells, and reduced the SARS-CoV-2 pseudovirus entry to HEK293/hACE2 cells. Furthermore, molecular docking studies indicated that anisodamine may target SARS-CoV-2 main protease (Mpro) with the docking score of -6.63 kcal/mol and formed three H-bonds with Gly143, Cys145, and Cys44 amino acid residues at the predicted active site of Mpro. This study suggests that anisodamine is a potent antiviral agent for treating COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Solanaceous Alkaloids / Coronavirus 3C Proteases / SARS-CoV-2 / COVID-19 / COVID-19 Drug Treatment Type of study: Prognostic study Topics: Traditional medicine Limits: Humans Language: English Journal: Biochem Biophys Res Commun Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Solanaceous Alkaloids / Coronavirus 3C Proteases / SARS-CoV-2 / COVID-19 / COVID-19 Drug Treatment Type of study: Prognostic study Topics: Traditional medicine Limits: Humans Language: English Journal: Biochem Biophys Res Commun Year: 2022 Document Type: Article