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Quantitative, multiplexed, targeted proteomics for ascertaining variant specific SARS-CoV-2 antibody response.
Doykov, Ivan; Baldwin, Tomas; Spiewak, Justyna; Gilmour, Kimberly C; Gibbons, Joseph M; Pade, Corinna; Reynolds, Catherine J; Noursadeghi, Mahdad; Maini, Mala K; Manisty, Charlotte; Treibel, Thomas; Captur, Gabriella; Fontana, Marianna; Boyton, Rosemary J; Altmann, Daniel M; Brooks, Tim; Semper, Amanda; Moon, James C; Heywood, Wendy E.
  • Doykov I; Translational Mass Spectrometry Research Group, Genetics & Genomic Medicine Department, UCL Institute of Child Health, London, UK.
  • Baldwin T; Great Ormond Street Biomedical Research Centre, UCL Institute of Child Health London.
  • Spiewak J; Translational Mass Spectrometry Research Group, Genetics & Genomic Medicine Department, UCL Institute of Child Health, London, UK.
  • Gilmour KC; Translational Mass Spectrometry Research Group, Genetics & Genomic Medicine Department, UCL Institute of Child Health, London, UK.
  • Gibbons JM; Great Ormond Street Children's Hospital NHS Foundation Trust, Great Ormond Street, London WC1N 3JH, UK.
  • Pade C; Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Reynolds CJ; Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Áine McKnight; Department of Infectious Disease, Imperial College London, London, UK.
  • Noursadeghi M; Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Maini MK; Division of Infection and Immunity, University College London, London, UK.
  • Manisty C; Division of Infection and Immunity, University College London, London, UK.
  • Treibel T; St. Bartholomew's Hospital, Barts Health NHS Trust, London, UK.
  • Captur G; Institute of Cardiovascular Science, University College London, London, UK.
  • Fontana M; St. Bartholomew's Hospital, Barts Health NHS Trust, London, UK.
  • Boyton RJ; Institute of Cardiovascular Science, University College London, London, UK.
  • Altmann DM; Institute of Cardiovascular Science, University College London, London, UK.
  • Brooks T; Royal Free London NHS Foundation Trust, Pond Street, London NW3 2QG, UK.
  • Semper A; Institute of Cardiovascular Science, University College London, London, UK.
  • Moon JC; Department of Infectious Disease, Imperial College London, London, UK.
  • Kevin Mills; Lung Division, Royal Brompton and Harefield Hospitals, Guy's and St Thomas' NHS Foundation Trust, London, UK.
  • Heywood WE; Department of Immunology and Inflammation, Imperial College London, London, UK.
Cell Rep Methods ; 2(9): 100279, 2022 Sep 19.
Article in English | MEDLINE | ID: covidwho-1982890
ABSTRACT
Determining the protection an individual has to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants of concern (VoCs) is crucial for future immune surveillance, vaccine development, and understanding of the changing immune response. We devised an informative assay to current ELISA-based serology using multiplexed, baited, targeted proteomics for direct detection of multiple proteins in the SARS-CoV-2 anti-spike antibody immunocomplex. Serum from individuals collected after infection or first- and second-dose vaccination demonstrates this approach and shows concordance with existing serology and neutralization. Our assays show altered responses of both immunoglobulins and complement to the Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.1) VoCs and a reduced response to Omicron (B1.1.1529). We were able to identify individuals who had prior infection, and observed that C1q is closely associated with IgG1 (r > 0.82) and may better reflect neutralization to VoCs. Analyzing additional immunoproteins beyond immunoglobulin (Ig) G, provides important information about our understanding of the response to infection and vaccination.
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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines / Variants Language: English Journal: Cell Rep Methods Year: 2022 Document Type: Article Affiliation country: J.crmeth.2022.100279

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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines / Variants Language: English Journal: Cell Rep Methods Year: 2022 Document Type: Article Affiliation country: J.crmeth.2022.100279