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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulins using chemiluminescence immunoassay and its correlation with neutralizing antibodies.
Gaber, Yasmine; Abdel Alem, Shereen; Musa, Sherief; Amer, Khaled; Elnagdy, Tarek; Hassan, Wael A; Abdelrahman, Raafat Zaher; Gad, Ahmed; Ali, Mohamed A; Badary, Hedy A; Shawky, Shereen; Talaat, Hala; Kassem, Abdel Meguid; Fouad, Rabab.
  • Gaber Y; Endemic Medicine Department, Kasr Al-Aini Faculty of Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt. Electronic address: yasmin.mohamed@cu.edu.eg.
  • Abdel Alem S; Endemic Medicine Department, Kasr Al-Aini Faculty of Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Musa S; Endemic Medicine Department, Kasr Al-Aini Faculty of Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Amer K; Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo, Egypt.
  • Elnagdy T; Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo, Egypt.
  • Hassan WA; Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo, Egypt.
  • Abdelrahman RZ; Armed Forces Laboratories for Medical Research and Blood Bank, Egypt.
  • Gad A; Armed Forces Laboratories for Medical Research and Blood Bank, Egypt.
  • Ali MA; Center of Scientific Excellence for Influenza Viruses, National Research Center, Giza, Egypt.
  • Badary HA; Endemic Medicine Department, Kasr Al-Aini Faculty of Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Shawky S; Clinical Pathology Department, Kasr Al-Aini Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Talaat H; Department of Pediatrics, Kasr Al-Aini Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Kassem AM; Endemic Medicine Department, Kasr Al-Aini Faculty of Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Fouad R; Endemic Medicine Department, Kasr Al-Aini Faculty of Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.
Virus Res ; 319: 198852, 2022 Oct 02.
Article in English | MEDLINE | ID: covidwho-1984220
ABSTRACT

BACKGROUND:

Neutralizing antibodies (NAbs) against SARS-CoV-2 infection have a pivotal role in protective immune response; however, their measurement requires specialized facilities. We evaluated the degree of correlation between NAbs and anti-SARS-CoV-2 IgG/total Ig antibodies detected by chemiluminescent immunoassay in asymptomatic and previously symptomatic SARS-CoV-2 patients.

METHODS:

A total of 1241 participants (previously symptomatic patients and asymptomatic individuals), who were screened for SARS-CoV-2 infection by RT-PCR or serology, were enrolled in our study. Sera were analyzed for the presence of anti-spike-1(S1)-SARS-CoV-2 IgG/total Ig antibodies, using Ortho Clinical Diagnostics, USA. A signal/cut-off value (S/CO) ≥ 1 was considered reactive. NAbs were measured in 103 random samples from groups using microneutralization assay, with titer ≥ 110 being considered positive.

RESULTS:

Asymptomatic (n = 229) and 261 previously symptomatic individuals with positive serology and negative RT-PCR were finally included. Significant higher anti-S1-IgG titers were seen in asymptomatic individuals (P < 0.0001). Conversely, anti-S1-total Ig titers were significantly higher in previously symptomatic (P < 0.0001). NAbs were detected in both groups, however, higher titers were seen in previously symptomatic patients. There is a correlation between NAbs and both IgG/total anti-S1-SARS-CoV-2 antibodies (r = 0.47, P < 0.0001 and r = 0.49, P < 0.0001, respectively). IgG and total Ig could predict a neutralization titer of ≥ 1160 at S/CO >4.44 and >65 with AUC 0.69 and 0.67, respectively.

CONCLUSION:

Asymptomatic SARS-CoV-2 infection can produce comparable antibodies response to previously symptomatic individuals, however higher neutralization activity was seen in the previously symptomatic. Anti-S1-SARS-CoV-2 IgG/total Ig antibodies showed a correlation with neutralization activity and can be used to estimate the presence of protective immunity.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study / Randomized controlled trials Topics: Variants Limits: Humans Language: English Journal: Virus Res Journal subject: Virology Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study / Randomized controlled trials Topics: Variants Limits: Humans Language: English Journal: Virus Res Journal subject: Virology Year: 2022 Document Type: Article