Platinum-Decorated Gold Nanoparticle-Based Microfluidic Chip Immunoassay for Ultrasensitive Colorimetric Detection of SARS-CoV-2 Nucleocapsid Protein.
ACS Biomater Sci Eng
; 8(9): 3924-3932, 2022 09 12.
Article
in English
| MEDLINE | ID: covidwho-1984354
ABSTRACT
Gold nanoparticle-based point-of-care tests (POCT) are one of the most widely used diagnostic tools for SARS-CoV-2 screening. However, the limitation of their insufficient sensitivity often leads to false negative results in early disease diagnostics. The ongoing pandemic of COVID-19 makes diagnostic tools that are more accurate, sensitive, simple, and affordable in high demand. In this work, we develop a platinum-decorated gold nanoparticle (Au@Pt NP)-based microfluidic chip immunoassay with a sensitivity surpassing that of paper-based detection of nucleocapsid (N) protein, one of the most conserved biomarkers of COVID-19. The synthesized Au@Pt NPs show high stability and catalytic activity in complex environments. The catalytic amplification of Au@Pt NPs enables naked-eye detection of N protein in the low femtogram range (ca. 0.1 pg/mL) and the detection of throat swab samples in under 40 min. This microfluidic chip immunoassay is easy for operation and readout without instrument assistance, making it more suitable for on-site detection and future pathogen surveillance.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Metal Nanoparticles
/
COVID-19
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
ACS Biomater Sci Eng
Year:
2022
Document Type:
Article
Affiliation country:
Acsbiomaterials.2c00600
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