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A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2.
Chen, Zhi; Li, Jingfeng; Li, Tianzhong; Fan, Taojian; Meng, Changle; Li, Chaozhou; Kang, Jianlong; Chai, Luxiao; Hao, Yabin; Tang, Yuxuan; Al-Hartomy, Omar A; Wageh, Swelm; Al-Sehemi, Abdullah G; Luo, Zhiguang; Yu, Jiangtian; Shao, Yonghong; Li, Defa; Feng, Shuai; Liu, William J; He, Yaqing; Ma, Xiaopeng; Xie, Zhongjian; Zhang, Han.
  • Chen Z; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
  • Li J; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
  • Li T; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
  • Fan T; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
  • Meng C; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
  • Li C; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
  • Kang J; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
  • Chai L; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
  • Hao Y; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
  • Tang Y; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
  • Al-Hartomy OA; Department of Physics, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Wageh S; Department of Physics, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Al-Sehemi AG; Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha 61413, Saudi Arabia.
  • Luo Z; Zhongmin (Shenzhen) Intelligent Ecology Co. Ltd, Shenzhen 518055, China.
  • Yu J; Shenzhen International Institute for Biomedical Research, Shenzhen 518116, China.
  • Shao Y; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
  • Li D; Department of Laboratory Medicine, Shenzhen Children's Hospital, Shenzhen 518038, China.
  • Feng S; Optoelectronics Research Center, School of Science, Minzu University of China, Beijing 100081, China.
  • Liu WJ; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • He Y; Institute of Pathogenic Organism, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
  • Ma X; Respiratory Department , Shenzhen Children's Hospital, Shenzhen 518038, China.
  • Xie Z; Institute of Pediatrics, Shenzhen Children's Hospital, Shenzhen 518038, China.
  • Zhang H; Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics; International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education; Shenzhen Institute of Translational Medicine; Department of Otolaryngology, Shenzhen Second People's Hospita
Natl Sci Rev ; 9(8): nwac104, 2022 Aug.
Article in English | MEDLINE | ID: covidwho-1985098
ABSTRACT
The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-CoV-2 variants, including Omicron, are required to be identified as soon as the samples are identified as 'positive'. Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time, we have developed MOPCS (Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance (SPR) with the 'gene scissors' clustered regularly interspaced short palindromic repeat (CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-system-empowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification, within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-CoV-2, and mutations appear in variants B.1.617.2 (Delta), B.1.1.529 (Omicron) and BA.1 (a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Topics: Variants Language: English Journal: Natl Sci Rev Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Topics: Variants Language: English Journal: Natl Sci Rev Year: 2022 Document Type: Article