Microarray-Based Transcriptome Analysis of Peripheral Blood Mononuclear Cells in Lung Cancer Patients
Russian Journal of Genetics
; 58(7):814-822, 2022.
Article
in English
| EMBASE | ID: covidwho-1986344
ABSTRACT
Lung cancer is the most commonly occurring cancer in men worldwide. To search for new biological markers of this pathology, the transcriptome of the blood mononuclear cells from patients and healthy donors (residents of Kemerovo oblast, Russia) was studied using SurePrint G3 Human Gene Expression microarray technology. A total of 288 differentially expressed genes were identified, including 108 up-regulated genes and 180 down-regulated genes. Functional enrichment analysis using the WebGestalt resource and different databases (Gene Ontology, KEGG, and Reactome) indicated changes in the expression profiles of genes involved in the processes of immune response, protein synthesis, cell cycle control, and apoptosis. Analysis of protein–protein interactions using the STRING algorithm made it possible to identify functional clusters of gene products with different expression levels.
array scanner; RNA purification kit; chaperonin containing TCP1; dyskerin; adult; apoptosis; article; bioinformatics; cancer patient; carcinogenesis; cell cycle regulation; controlled study; coronavirus disease 2019; differential expression analysis; DNA damage; DNA microarray; donor; endoplasmic reticulum; false discovery rate; functional enrichment analysis; gene expression profiling; gene ontology; human; human cell; human experiment; immune response; KEGG; lung cancer; middle aged; osteoclast; pathology; peripheral blood mononuclear cell; phagosome; protein interaction; protein synthesis; ribosome; RNA extraction; TCR signaling; transcriptomics; tuberculosis
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Language:
English
Journal:
Russian Journal of Genetics
Year:
2022
Document Type:
Article
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