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Assessing the use of a micro-sampling device for measuring blood protein levels in healthy subjects and COVID-19 patients.
Brandsma, Joost; Chenoweth, Josh G; Gregory, Melissa K; Krishnan, Subramaniam; Blair, Paul W; Striegel, Deborah A; Mehta, Rittal; Schully, Kevin L; Dumler, J Stephen; Sikorski, Cdr Cynthia S; O'Connor, Kelsey; Reichert-Scrivner, Susan A; Paguirigan, Carmen M; Uyehara, Catherine F T; Ngauy, Col Viseth; Myers, Christopher A; Clark, Danielle V.
  • Brandsma J; Austere Environments Consortium for Enhanced Sepsis Outcomes, Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., Bethesda, Maryland, United States of America.
  • Chenoweth JG; Austere Environments Consortium for Enhanced Sepsis Outcomes, Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., Bethesda, Maryland, United States of America.
  • Gregory MK; Austere Environments Consortium for Enhanced Sepsis Outcomes, Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., Bethesda, Maryland, United States of America.
  • Krishnan S; Austere Environments Consortium for Enhanced Sepsis Outcomes, Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., Bethesda, Maryland, United States of America.
  • Blair PW; Austere Environments Consortium for Enhanced Sepsis Outcomes, Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., Bethesda, Maryland, United States of America.
  • Striegel DA; Department of Pathology, School of Medicine, Uniformed Services University, Bethesda, Maryland, United States of America.
  • Mehta R; Austere Environments Consortium for Enhanced Sepsis Outcomes, Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., Bethesda, Maryland, United States of America.
  • Schully KL; Austere Environments Consortium for Enhanced Sepsis Outcomes, Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., Bethesda, Maryland, United States of America.
  • Dumler JS; Austere Environments Consortium for Enhanced Sepsis Outcomes, Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Frederick, Maryland, United States of America.
  • Sikorski CCS; Department of Pathology, School of Medicine, Uniformed Services University, Bethesda, Maryland, United States of America.
  • O'Connor K; Naval Health Research Center, San Diego, California, United States of America.
  • Reichert-Scrivner SA; Austere Environments Consortium for Enhanced Sepsis Outcomes, Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., Bethesda, Maryland, United States of America.
  • Paguirigan CM; Tripler Army Medical Center, Honolulu, Hawaii, United States of America.
  • Uyehara CFT; Austere Environments Consortium for Enhanced Sepsis Outcomes, Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., Bethesda, Maryland, United States of America.
  • Ngauy CV; Tripler Army Medical Center, Honolulu, Hawaii, United States of America.
  • Myers CA; Tripler Army Medical Center, Honolulu, Hawaii, United States of America.
  • Clark DV; Tripler Army Medical Center, Honolulu, Hawaii, United States of America.
PLoS One ; 17(8): e0272572, 2022.
Article in English | MEDLINE | ID: covidwho-1987158
ABSTRACT

BACKGROUND:

Venous phlebotomy performed by trained personnel is critical for patient diagnosis and monitoring of chronic disease, but has limitations in resource-constrained settings, and represents an infection control challenge during outbreaks. Self-collection devices have the potential to shift phlebotomy closer to the point of care, supporting telemedicine strategies and virtual clinical trials. Here we assess a capillary blood micro-sampling device, the Tasso Serum Separator Tube (SST), for measuring blood protein levels in healthy subjects and non-hospitalized COVID-19 patients.

METHODS:

57 healthy controls and 56 participants with mild/moderate COVID-19 were recruited at two U.S. military healthcare facilities. Healthy controls donated Tasso SST capillary serum, venous plasma and venous serum samples at multiple time points, while COVID-19 patients donated a single Tasso SST serum sample at enrolment. Concentrations of 17 protein inflammatory biomarkers were measured in all biospecimens by Ella multi-analyte immune-assay.

RESULTS:

Tasso SST serum protein measurements in healthy control subjects were highly reproducible, but their agreements with matched venous samples varied. Most of the selected proteins, including CRP, Ferritin, IL-6 and PCT, were well-correlated between Tasso SST and venous serum with little sample type bias, but concentrations of D-dimer, IL-1B and IL-1Ra were not. Self-collection at home with delayed sample processing was associated with significant concentrations differences for several analytes compared to supervised, in-clinic collection with rapid processing. Finally, Tasso SST serum protein concentrations were significantly elevated in in non-hospitalized COVID-19 patients compared with healthy controls.

CONCLUSIONS:

Self-collection of capillary blood with micro-sampling devices provides an attractive alternative to routine phlebotomy. However, concentrations of certain analytes may differ significantly from those in venous samples, and factors including user proficiency, temperature control and time lags between specimen collection and processing need to be considered for their effect on sample quality and reproducibility.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: PLoS One Journal subject: Science / Medicine Year: 2022 Document Type: Article Affiliation country: Journal.pone.0272572

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: PLoS One Journal subject: Science / Medicine Year: 2022 Document Type: Article Affiliation country: Journal.pone.0272572