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A potent virucidal activity of functionalized TiO2 nanoparticles adsorbed with flavonoids against SARS-CoV-2.
León-Gutiérrez, Gabriela; Elste, James Edward; Cabello-Gutiérrez, Carlos; Millán-Pacheco, Cesar; Martínez-Gómez, Mario H; Mejía-Alvarez, Rafael; Tiwari, Vaibhav; Mejía, Armando.
  • León-Gutiérrez G; Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de Mexico, Mexico.
  • Elste JE; Department of Microbiology and Immunology, Midwestern University, Downers Grove, IL, USA.
  • Cabello-Gutiérrez C; Departamento de Virología e Investigación en Micología, Instituto Nacional de Enfermedades Respiratorias, Ciudad de Mexico, Mexico.
  • Millán-Pacheco C; Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
  • Martínez-Gómez MH; Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de Mexico, Mexico.
  • Mejía-Alvarez R; Department of Physiology, College of Graduate Studies, Midwestern University, Downers Grove, IL, USA.
  • Tiwari V; Department of Microbiology and Immunology, Midwestern University, Downers Grove, IL, USA.
  • Mejía A; Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de Mexico, Mexico. ama@xanum.uam.mx.
Appl Microbiol Biotechnol ; 106(18): 5987-6002, 2022 Sep.
Article in English | MEDLINE | ID: covidwho-1990606
ABSTRACT
The coronavirus SARS-CoV-2 has caused a pandemic with > 550 millions of cases and > 6 millions of deaths worldwide. Medical management of COVID-19 relies on supportive care as no specific targeted therapies are available yet. Given its devastating effects on the economy and mental health, it is imperative to develop novel antivirals. An ideal candidate will be an agent that blocks the early events of viral attachment and cell entry, thereby preventing viral infection and spread. This work reports functionalized titanium dioxide (TiO2)-based nanoparticles adsorbed with flavonoids that block SARS-CoV-2 entry and fusion. Using molecular docking analysis, two flavonoids were chosen for their specific binding to critical regions of the SARS-CoV-2 spike glycoprotein that interacts with the host cell angiotensin-converting enzyme-2 (ACE-2) receptor. These flavonoids were adsorbed onto TiO2 functionalized nanoparticles (FTNP). This new nanoparticulate compound was assayed in vitro against two different coronaviruses; HCoV 229E and SARS-CoV-2, in both cases a clear antiviral effect was observed. Furthermore, using a reporter-based cell culture model, a potent antiviral activity is demonstrated. The adsorption of flavonoids to functionalized TiO2 nanoparticles induces a ~ threefold increase of that activity. These studies also indicate that FTNP interferes with the SARS-CoV-2 spike, impairing the cell fusion mechanism. KEY POINTS/HIGHLIGHTS • Unique TiO2 nanoparticles displaying flavonoid showed potent anti-SARS-CoV-2 activity. • The nanoparticles precisely targeting SARS-CoV-2 were quantitatively verified by cell infectivity in vitro. • Flavonoids on nanoparticles impair the interactions between the spike glycoprotein and ACE-2 receptor.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nanoparticles / COVID-19 Drug Treatment Limits: Humans Language: English Journal: Appl Microbiol Biotechnol Year: 2022 Document Type: Article Affiliation country: S00253-022-12112-9

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nanoparticles / COVID-19 Drug Treatment Limits: Humans Language: English Journal: Appl Microbiol Biotechnol Year: 2022 Document Type: Article Affiliation country: S00253-022-12112-9