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Combining Community Wastewater Genomic Surveillance with State Clinical Surveillance: A Framework for SARS-CoV-2 Public Health Practice.
Smith, Ted; Holm, Rochelle H; Yeager, Ray; Moore, Joseph B; Rouchka, Eric C; Sokoloski, Kevin J; Elliott, Erin M; Talley, Daymond; Arora, Vaneet; Moyer, Sarah; Bhatnagar, Aruni.
  • Smith T; Christina Lee Brown Envirome Institute, School of Medicine, University of Louisville, 302 E. Muhammad Ali Blvd., Louisville, KY, 40202, USA.
  • Holm RH; Christina Lee Brown Envirome Institute, School of Medicine, University of Louisville, 302 E. Muhammad Ali Blvd., Louisville, KY, 40202, USA. rochelle.holm@louisville.edu.
  • Yeager R; Christina Lee Brown Envirome Institute, School of Medicine, University of Louisville, 302 E. Muhammad Ali Blvd., Louisville, KY, 40202, USA.
  • Moore JB; Center for Integrative Environmental Health Sciences, School of Medicine, University of Louisville, 500 S. Preston St., Louisville, KY, 40202, USA.
  • Rouchka EC; Christina Lee Brown Envirome Institute, School of Medicine, University of Louisville, 302 E. Muhammad Ali Blvd., Louisville, KY, 40202, USA.
  • Sokoloski KJ; Diabetes and Obesity Center, School of Medicine, University of Louisville, 580 S. Preston St., Louisville, KY, 40202, USA.
  • Elliott EM; Department of Biochemistry and Molecular Genetics, School of Medicine, University of Louisville, 319 Abraham Flexner Way, Louisville, KY, 40202, USA.
  • Talley D; Department of Microbiology and Immunology, School of Medicine, University of Louisville, 505 S. Hancock St., Louisville, KY, 40202, USA.
  • Arora V; Center for Predictive Medicine for Biodefense and Emerging Infectious Diseases, University of Louisville, 505 S. Hancock St., Louisville, KY, 40202, USA.
  • Moyer S; Christina Lee Brown Envirome Institute, School of Medicine, University of Louisville, 302 E. Muhammad Ali Blvd., Louisville, KY, 40202, USA.
  • Bhatnagar A; Diabetes and Obesity Center, School of Medicine, University of Louisville, 580 S. Preston St., Louisville, KY, 40202, USA.
Food Environ Virol ; 14(4): 410-416, 2022 Dec.
Article in English | MEDLINE | ID: covidwho-1990792
ABSTRACT
This study aimed to develop a framework for combining community wastewater surveillance with state clinical surveillance for the confirmation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants within the community and to provide recommendations on how to expand on such research and apply the findings in public health responses. Wastewater samples were collected weekly from 17 geographically resolved locations in Louisville/Jefferson County, Kentucky (USA), from February 10 to December 13, 2021. Genomic surveillance and quantitative reverse transcription PCR (RT-qPCR) platforms were used to screen for SARS-CoV-2 in wastewater, and state clinical surveillance was used for confirmation. The study results highlighted an increased epidemiological value of combining community wastewater genomic surveillance and RT-qPCR with conventional case-auditing methods. The spatial scale and temporal frequency of wastewater sampling provided promising sensitivity and specificity for gaining public health screening insights about SARS-CoV-2 emergence, seeding, and spread in communities. Improved national surveillance systems are needed against future pathogens and variants, and wastewater-based genomic surveillance exhibits great potential when coupled with clinical testing. This paper presents evidence that complementary wastewater and clinical testing are cost-effectively enhanced when used in combination, as they provide a strong tool for a joint public health framework. Future pathogens of interest may be examined in either a targeted fashion or using a more global approach where all pathogens are monitored. This study has also provided novel insights developed from evidence-based public health practices.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Topics: Variants Limits: Humans Language: English Journal: Food Environ Virol Year: 2022 Document Type: Article Affiliation country: S12560-022-09531-2

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Topics: Variants Limits: Humans Language: English Journal: Food Environ Virol Year: 2022 Document Type: Article Affiliation country: S12560-022-09531-2