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A multiplex assay for the detection of antibodies to relevant swine pathogens in serum.
Aira, Cristina; Penning, Maren; Eiden, Martin; Balkema-Buschmann, Anne; Blome, Sandra; Strutzberg-Minder, Katrin; López, Lissette; Rueda, Paloma; Sastre, Patricia.
  • Aira C; Research Department, Eurofins-Ingenasa, Madrid, Spain.
  • Penning M; Friedrich-Loeffler Institute (FLI), Institute of Novel and Emerging Infectious Diseases (INNT), Greifswald-Insel Riems, Germany.
  • Eiden M; Friedrich-Loeffler Institute (FLI), Institute of Novel and Emerging Infectious Diseases (INNT), Greifswald-Insel Riems, Germany.
  • Balkema-Buschmann A; Friedrich-Loeffler Institute (FLI), Institute of Novel and Emerging Infectious Diseases (INNT), Greifswald-Insel Riems, Germany.
  • Blome S; Friedrich-Loeffler Institute (FLI), Institute Diagnostic Virology (IVD), Greifswald-Insel Riems, Germany.
  • Strutzberg-Minder K; IVD Gesellschaft für Innovative Veterinärdiagnostik mbH (IVD GmbH), Seelze, Germany.
  • López L; Research Department, Eurofins-Ingenasa, Madrid, Spain.
  • Rueda P; Research Department, Eurofins-Ingenasa, Madrid, Spain.
  • Sastre P; Research Department, Eurofins-Ingenasa, Madrid, Spain.
Transbound Emerg Dis ; 69(4): 2173-2181, 2022 Jul.
Article in English | MEDLINE | ID: covidwho-1992901
ABSTRACT
Livestock industry supports the livelihood of around 1.3 billion people in the world, with swine industry contributing with 30% of total livestock production worldwide. To maintain and guarantee this production, a pivotal point according to the OIE is addressing potential biohazards. To control them, permanent sero-surveillance is crucial to achieve more focused veterinary public health intervention and prevention strategies, to break the chains of transmission, and to enable fast responses against outbreaks. Within this context, multiplex assays are powerful tools with the potential to simplify surveillance programs, since they reduce time, labour, and variability within analysis. In the present work, we developed a multiplex bead-based assay for the detection of specific antibodies to six relevant pathogens affecting swine ASFV, CSFV, PRRSV, SIV, TB and HEV. The most immunogenic target antigen of each pathogen was selected as the target protein to coat different microsphere regions in order to develop this multiplex assay. A total of 1544 serum samples from experimental infections as well as field samples were included in the analysis. The 6-plex assay exhibited credible diagnostic parameters with sensitivities ranging from 87.0% to 97.5% and specificities ranging from 87.9% to 100.0%, demonstrating it to be a potential high throughput tool for surveillance of infectious diseases in swine.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Swine Diseases / Porcine respiratory and reproductive syndrome virus / African Swine Fever / African Swine Fever Virus Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: Transbound Emerg Dis Journal subject: Veterinary Medicine Year: 2022 Document Type: Article Affiliation country: Tbed.14213

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Swine Diseases / Porcine respiratory and reproductive syndrome virus / African Swine Fever / African Swine Fever Virus Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: Transbound Emerg Dis Journal subject: Veterinary Medicine Year: 2022 Document Type: Article Affiliation country: Tbed.14213