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Establishment and application of semi-nest RT-PCR for detection of Transmissible gastroenteritis virus
Chinese Veterinary Science / Zhongguo Shouyi Kexue ; 50(5):556-562, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-1994650
ABSTRACT
In order to establish an efficient, sensitive and specific semi-nest RT-PCR method for the detection of Transmissible gastroenteritis virus (TGEV), three specific primers were designed according to the N gene published by GenBank, the reaction system was established and optimized, and specificity and sensitivity were detected. The results showed that the method could successfully amplify the bands of 483 bp and 338 bp, and had good specificity to TGEV, there is no cross reaction with PEDV, PRov, PBov and PDCov, and the lowest sensitivity was 1.86 x 10-1 pg/L. The semi-nest RT-PCR shown the positive rate was 36% in 50 samples of pig diarrhea, which was higher than that of common RT-PCR, and then the positive samples coincidence rate was 100%. This semi-nest RT-PCR method has high sensitivity and specificity, and can accurately diagnose TGEV infection, which provides an effective method for clinical detection and epidemiological investigation of TGEV.
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Full text: Available Collection: Databases of international organizations Database: CAB Abstracts Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Language: Chinese Journal: Zhongguo Shouyi Kexue Year: 2020 Document Type: Article

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Full text: Available Collection: Databases of international organizations Database: CAB Abstracts Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Language: Chinese Journal: Zhongguo Shouyi Kexue Year: 2020 Document Type: Article