Effects of lncRNA9606 on porcine epidemic diarrhea virus replication
Chinese Veterinary Science / Zhongguo Shouyi Kexue
; 50(5):537-544, 2020.
Article
in Chinese
| CAB Abstracts | ID: covidwho-1994651
ABSTRACT
Long noncoding RNA (lncRNA) is a type of non-coding RNA molecule longer than 200 nt, which plays vital roles in biological events. Our previous results demonstrated that the host's lncRNA expression profile was significantly changed after porcine epidemic diarrhea virus (PEDV) infection. In this study, one of the lncRNAs, lncRNA9606, was selected to investigate its impact on PEDV replication. First, the kinetics of lncRNA9606 expression in IPEC-J2 cells were examined at different time points after PEDV infection. The results confirmed that PEDV infection significantly upregulated the expression of lncRNA9606. The lncRNA9606 expression levels in different cells or tissues were evaluated and the results showed that the amount of lncRNA9606 in Peyer's patches and peripheral blood mononuclear cells were significantly higher than that in small intestinal epithelial cell lines. It was mainly localized in the nucleus. Further investigations indicated that over expression of lncRNA in LLC-PK1 cells significantly inhibited PEDV replication. In conclusion, lncRNA9606 can suppress the PEDV replication in LLC-PK1 cells.
Meat Producing Animals [LL120]; Prion, Viral, Bacterial and Fungal Pathogens of Animals [LL821]; Molecular Biology and Molecular Genetics [ZZ360]; Biochemistry and Physiology of Microorganisms [ZZ394]; Genetics and Molecular Biology of Microorganisms [ZZ395]; cell lines; cells; cytopathogenicity; epithelium; gene expression; porcine epidemic diarrhoea; RNA; small intestine; viral replication; pigs; Porcine epidemic diarrhea virus; Sus scrofa; Sus; Suidae; Suiformes; Artiodactyla; mammals; vertebrates; Chordata; animals; eukaryotes; Alphacoronavirus; Coronavirinae; Coronaviridae; Nidovirales; positive-sense ssRNA Viruses; ssRNA Viruses; RNA Viruses; viruses; swine; hogs; Porcine epidemic diarrhoea virus; ribonucleic acid
Full text:
Available
Collection:
Databases of international organizations
Database:
CAB Abstracts
Type of study:
Experimental Studies
Language:
Chinese
Journal:
Zhongguo Shouyi Kexue
Year:
2020
Document Type:
Article
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