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Diagnostic performance of the SARS-CoV-2 S1RBD IgG ELISA (ImmunoDiagnostics) for the quantitative detection of SARS-CoV-2 antibodies on dried blood spots.
Meyers, Eline; Coen, Anja; De Sutter, An; Padalko, Elizaveta; Callens, Steven; Vandekerckhove, Linos; Witkowski, Wojciech; Heytens, Stefan; Cools, Piet.
  • Meyers E; Laboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium. Electronic address: eline.meyers@ugent.be.
  • Coen A; Department of Public Health and Primary Care, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium. Electronic address: anja.coen@ugent.be.
  • De Sutter A; Department of Public Health and Primary Care, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium. Electronic address: an.desutter@ugent.be.
  • Padalko E; Laboratory of Medical Microbiology, Ghent University Hospital, 9000 Ghent, Belgium. Electronic address: elizaveta.padalko@uzgent.be.
  • Callens S; Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium. Electronic address: steven.callens@ugent.be.
  • Vandekerckhove L; HIV Cure Research Centre, Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium. Electronic address: linos.vandekerckhove@ugent.be.
  • Witkowski W; HIV Cure Research Centre, Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium. Electronic address: wojciech.witkowski@ugent.be.
  • Heytens S; Department of Public Health and Primary Care, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium. Electronic address: stefan.heytens@ugent.be.
  • Cools P; Laboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium. Electronic address: piet.cools@ugent.be.
J Clin Virol ; 155: 105270, 2022 10.
Article in English | MEDLINE | ID: covidwho-1996329
ABSTRACT
Dried Blood Spots (DBS) are broadly used in SARS-CoV-2 surveillance studies, reporting either the presence or absence of SARS-CoV-2 antibodies. However, quantitative follow-up has become increasingly important to monitor humoral vaccine responses. Therefore, we aimed to evaluate the performance of DBS for the detection of anti-spike SARS-CoV-2 antibody concentrations using a commercially available assay, reporting in a standardised unitage (International Units/mL; IU/mL). To assess the sensitivity and specificity of the ImmunoDiagnostics ELISA on serum and DBS for SARS-CoV-2 antibody detection, we analysed 72 paired DBS and serum samples. The SARS-CoV-2 S1 IgG ELISA kit (EUROIMMUN) on serum was used as the reference method. We performed a statistical assessment to optimise the cut-off value for DBS and serum and assessed the correlation between DBS and serum antibody concentrations. We found that anti-spike SARS-CoV-2 antibody concentrations detected in DBS are highly correlated to those detected in paired serum (Pearson correlation 0.98; p-value < 0.0001), allowing to assess serum antibody concentration using DBS. The optimal cut-off for antibody detection on DBS was found to be 26 IU/mL, with 98.1% sensitivity and 100% specificity. For serum, the optimal cut-off was 14 IU/mL, with 100% sensitivity and 100% specificity. Therefore, we conclude that the ImmunoDiagnostics ELISA kit has optimal performance in the detection of SARS-CoV-2 antibodies on both DBS and serum. This makes DBS ideal for large-scale follow-up of humoral SARS-CoV-2 immune responses, as it is an easy but valuable sampling method for quantification of SARS-CoV-2 antibodies, compared to serum.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Experimental Studies / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: J Clin Virol Journal subject: Virology Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Experimental Studies / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: J Clin Virol Journal subject: Virology Year: 2022 Document Type: Article