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Integration of Multiple Interferometers in Highly Multiplexed Diagnostic KITs to Evaluate Several Biomarkers of COVID-19 in Serum.
Murillo, Ana María M; Valle, Luis G; Ramírez, Yolanda; Sánchez, María Jesús; Santamaría, Beatriz; Molina-Roldan, E; Ortega-Madueño, Isabel; Urcelay, Elena; Tramarin, Luca; Herreros, Pedro; Díaz-Perales, Araceli; Garrido-Arandia, María; Tome-Amat, Jaime; Hernández-Ramírez, Guadalupe; Espinosa, Rocío L; Laguna, María F; Holgado, Miguel.
  • Murillo AMM; Center for Biomedical Technology (CTB), Universidad Politécnica de Madrid, Parque Científico y Tecnológico de la UPM, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain.
  • Valle LG; Health Research Institute of the Hospital Clínico San Carlos, IdISSC. C/Profesor Martín Lagos s/n, 4ª Planta Sur, 28040 Madrid, Spain.
  • Ramírez Y; BioOptical Detection S.L., Centro de Empresas, Campus Montegancedo, 28223 Madrid, Spain.
  • Sánchez MJ; Center for Biomedical Technology (CTB), Universidad Politécnica de Madrid, Parque Científico y Tecnológico de la UPM, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain.
  • Santamaría B; Health Research Institute of the Hospital Clínico San Carlos, IdISSC. C/Profesor Martín Lagos s/n, 4ª Planta Sur, 28040 Madrid, Spain.
  • Molina-Roldan E; Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, C/José Gutiérrez Abascal 2, 28006 Madrid, Spain.
  • Ortega-Madueño I; Center for Biomedical Technology (CTB), Universidad Politécnica de Madrid, Parque Científico y Tecnológico de la UPM, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain.
  • Urcelay E; Health Research Institute of the Hospital Clínico San Carlos, IdISSC. C/Profesor Martín Lagos s/n, 4ª Planta Sur, 28040 Madrid, Spain.
  • Tramarin L; BioOptical Detection S.L., Centro de Empresas, Campus Montegancedo, 28223 Madrid, Spain.
  • Herreros P; Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, C/José Gutiérrez Abascal 2, 28006 Madrid, Spain.
  • Díaz-Perales A; Center for Biomedical Technology (CTB), Universidad Politécnica de Madrid, Parque Científico y Tecnológico de la UPM, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain.
  • Garrido-Arandia M; Health Research Institute of the Hospital Clínico San Carlos, IdISSC. C/Profesor Martín Lagos s/n, 4ª Planta Sur, 28040 Madrid, Spain.
  • Tome-Amat J; Escuela Técnica Superior de Ingeniería y Diseño Industrial, Universidad Politécnica de Madrid, Ronda de Valencia 3, 28012 Madrid, Spain.
  • Hernández-Ramírez G; Health Research Institute of the Hospital Clínico San Carlos, IdISSC. C/Profesor Martín Lagos s/n, 4ª Planta Sur, 28040 Madrid, Spain.
  • Espinosa RL; Health Research Institute of the Hospital Clínico San Carlos, IdISSC. C/Profesor Martín Lagos s/n, 4ª Planta Sur, 28040 Madrid, Spain.
  • Laguna MF; Health Research Institute of the Hospital Clínico San Carlos, IdISSC. C/Profesor Martín Lagos s/n, 4ª Planta Sur, 28040 Madrid, Spain.
  • Holgado M; Center for Biomedical Technology (CTB), Universidad Politécnica de Madrid, Parque Científico y Tecnológico de la UPM, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain.
Biosensors (Basel) ; 12(9)2022 Aug 23.
Article in English | MEDLINE | ID: covidwho-1997517
ABSTRACT
In the present work, highly multiplexed diagnostic KITs based on an Interferometric Optical Detection Method (IODM) were developed to evaluate six Coronavirus Disease 2019 (COVID-19)-related biomarkers. These biomarkers of COVID-19 were evaluated in 74 serum samples from severe, moderate, and mild patients with positive polymerase chain reaction (PCR), collected at the end of March 2020 in the Hospital Clínico San Carlos, in Madrid (Spain). The developed multiplexed diagnostic KITs were biofunctionalized to simultaneously measure different types of specific biomarkers involved in COVID-19. Thus, the serum samples were investigated by measuring the total specific Immunoglobulins (sIgT), specific Immunoglobulins G (sIgG), specific Immunoglobulins M (sIgM), specific Immunoglobulins A (sIgA), all of them against SARS-CoV-2, together with two biomarkers involved in inflammatory disorders, Ferritin (FER) and C Reactive Protein (CRP). To assess the results, a Multiple Linear Regression Model (MLRM) was carried out to study the influence of IgGs, IgMs, IgAs, FER, and CRP against the total sIgTs in these serum samples with a goodness of fit of 73.01% (Adjusted R-Squared).
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Testing / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Bios12090671

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Testing / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Year: 2022 Document Type: Article Affiliation country: Bios12090671