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Development of an indirect ELISA to detect PEDV specific IgA antibody based on a PEDV epidemic strain.
Wang, Kun; Hu, Zhiqiang; Fan, Mingyu; Shao, Zhenwen; Yu, Qiannan; Li, Xiaowen.
  • Wang K; Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd (NHLH Academy of Swine Research), Dezhou, China.
  • Hu Z; Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd (NHLH Academy of Swine Research), Dezhou, China.
  • Fan M; Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd (NHLH Academy of Swine Research), Dezhou, China.
  • Shao Z; Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd (NHLH Academy of Swine Research), Dezhou, China.
  • Yu Q; Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd (NHLH Academy of Swine Research), Dezhou, China.
  • Li X; Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd (NHLH Academy of Swine Research), Dezhou, China. lxw8272@163.com.
BMC Vet Res ; 18(1): 319, 2022 Aug 18.
Article in English | MEDLINE | ID: covidwho-2002179
ABSTRACT

BACKGROUND:

Porcine epidemic diarrhea (PED), a swine epidemic disease caused by porcine epidemic diarrhea virus (PEDV), is characterized by severe watery diarrhea, vomiting, dehydration and high mortality in piglets, and has caused serious economic losses to the global porcine industry. The level of PEDV IgA antibody is a key marker to assess the extent of passive immunity of the resistance against PEDV infection. However, current commercial structure proteins-based kits for detection of PEDV antibody are not affordable, and those kits require complicated antigen preparation procedures, which cannot meet the scope of economic benefits of many large-scale pig companies in China. Therefore, there is an urgent need to develop an accurate, simple, and economical method for IgA detection in clinical samples. In this study, an indirect ELISA (i-ELISA) method was developed based on a purified PEDV epidemic strain (NH-TA2020).

RESULTS:

The results show that optimal working dilution ratios of PEDV antigen and HRP anti-swine IgA are at 1 1000 and 115000 respectively. The sensitivity of this method is high with the maximum dilution of samples up to 1160, and coefficients of variation (CV) of both the intra assays and inter assays were no more than 15%. In addition, the relative sensitivities of the i-ELISA were above 90% compared with values from commercial kits in both serum and oral fluid samples.

CONCLUSIONS:

Our results suggested that the i-ELISA developed in this study was an accurate, simple, and economical method for PEDV-IgA detection in clinical samples.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Swine Diseases / Coronavirus Infections / Porcine epidemic diarrhea virus Type of study: Diagnostic study / Observational study / Prognostic study Limits: Animals Language: English Journal: BMC Vet Res Journal subject: Veterinary Medicine Year: 2022 Document Type: Article Affiliation country: S12917-022-03419-w

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Swine Diseases / Coronavirus Infections / Porcine epidemic diarrhea virus Type of study: Diagnostic study / Observational study / Prognostic study Limits: Animals Language: English Journal: BMC Vet Res Journal subject: Veterinary Medicine Year: 2022 Document Type: Article Affiliation country: S12917-022-03419-w