Public Cord Blood Banks as a source of starting material for clinical grade HLA-homozygous induced pluripotent stem cells.
Stem Cell Res Ther
; 13(1): 408, 2022 08 12.
Article
in English
| MEDLINE | ID: covidwho-2002225
ABSTRACT
BACKGROUND:
The increasing number of clinical trials for induced pluripotent stem cell (iPSC)-derived cell therapy products makes the production on clinical grade iPSC more and more relevant and necessary. Cord blood banks are an ideal source of young, HLA-typed and virus screened starting material to produce HLA-homozygous iPSC lines for wide immune-compatibility allogenic cell therapy approaches. The production of such clinical grade iPSC lines (haplolines) involves particular attention to all steps since donor informed consent, cell procurement and a GMP-compliant cell isolation process.METHODS:
Homozygous cord blood units were identified and quality verified before recontacting donors for informed consent. CD34+ cells were purified from the mononuclear fraction isolated in a cell processor, by magnetic microbeads labelling and separation columns.RESULTS:
We obtained a median recovery of 20.0% of the collected pre-freezing CD34+, with a final product median viability of 99.1% and median purity of 83.5% of the post-thawed purified CD34+ population.CONCLUSIONS:
Here we describe our own experience, from unit selection and donor reconsenting, in generating a CD34+ cell product as a starting material to produce HLA-homozygous iPSC following a cost-effective and clinical grade-compliant procedure. These CD34+ cells are the basis for the Spanish bank of haplolines envisioned to serve as a source of cell products for clinical research and therapy.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Induced Pluripotent Stem Cells
Type of study:
Prognostic study
Language:
English
Journal:
Stem Cell Res Ther
Year:
2022
Document Type:
Article
Affiliation country:
S13287-022-02961-6
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