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Molecular detection of SARS-COV-2 in exhaled breath at the point-of-need.
Stakenborg, Tim; Raymenants, Joren; Taher, Ahmed; Marchal, Elisabeth; Verbruggen, Bert; Roth, Sophie; Jones, Ben; Yurt, Abdul; Duthoo, Wout; Bombeke, Klaas; Fauvart, Maarten; Verplanken, Julien; Wiederkehr, Rodrigo S; Humbert, Aurelie; Dang, Chi; Vlassaks, Evi; Jáuregui Uribe, Alejandra L; Luo, Zhenxiang; Liu, Chengxun; Zinoviev, Kirill; Labie, Riet; Frederiks, Aduen Darriba; Saldien, Jelle; Covens, Kris; Berden, Pieter; Schreurs, Bert; Van Duppen, Joost; Hanifa, Rabea; Beuscart, Megane; Pham, Van; Emmen, Erik; Dewagtere, Annelien; Lin, Ziduo; Peca, Marco; El Jerrari, Youssef; Nawghane, Chinmay; Arnett, Chad; Lambrechts, Andy; Deshpande, Paru; Lagrou, Katrien; De Munter, Paul; André, Emmanuel; Van den Wijngaert, Nik; Peumans, Peter.
  • Stakenborg T; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Raymenants J; Department of Microbiology, Immunology and Transplantation, KU Leuven, Belgium; Department of General Internal Medicine, University Hospitals Leuven, Belgium.
  • Taher A; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Marchal E; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Verbruggen B; Imec Solutions Department, Imec, Leuven, Belgium.
  • Roth S; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Jones B; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Yurt A; Imec Solutions Department, Imec, Leuven, Belgium.
  • Duthoo W; Enabling Digital Transformations Department, Imec, Ghent, Belgium.
  • Bombeke K; Imec-mict-UGent, Department of Communication Sciences, Ghent University, Belgium.
  • Fauvart M; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Verplanken J; Enabling Digital Transformations Department, Imec, Ghent, Belgium.
  • Wiederkehr RS; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Humbert A; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Dang C; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Vlassaks E; Imec Solutions Department, Imec, Leuven, Belgium.
  • Jáuregui Uribe AL; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Luo Z; Imec Solutions Department, Imec, Leuven, Belgium.
  • Liu C; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Zinoviev K; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Labie R; Imec Solutions Department, Imec, Leuven, Belgium.
  • Frederiks AD; Imec-mict-UGent, Department of Industrial Systems Engineering and Product Design, Ghent University, Belgium.
  • Saldien J; Imec-mict-UGent, Department of Industrial Systems Engineering and Product Design, Ghent University, Belgium.
  • Covens K; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Berden P; Life Science Technologies Department, Imec, Leuven, Belgium; Department of Physics and Astronomy, KU Leuven, Belgium.
  • Schreurs B; Department of Business, Research Group Management and Strategy, Vrije Universiteit Brussel, Belgium.
  • Van Duppen J; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Hanifa R; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Beuscart M; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Pham V; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Emmen E; Imec Solutions Department, Imec, Leuven, Belgium.
  • Dewagtere A; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Lin Z; Imec Solutions Department, Imec, Leuven, Belgium.
  • Peca M; Life Science Technologies Department, Imec, Leuven, Belgium.
  • El Jerrari Y; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Nawghane C; Imec Solutions Department, Imec, Leuven, Belgium.
  • Arnett C; Imec Solutions Department, Imec, Leuven, Belgium.
  • Lambrechts A; Imec Solutions Department, Imec, Leuven, Belgium.
  • Deshpande P; Life Science Technologies Department, Imec, Leuven, Belgium.
  • Lagrou K; Department of Microbiology, Immunology and Transplantation, KU Leuven, Belgium; Department of Laboratory Medicine, University Hospitals Leuven, Belgium.
  • De Munter P; Department of Microbiology, Immunology and Transplantation, KU Leuven, Belgium; Department of General Internal Medicine, University Hospitals Leuven, Belgium.
  • André E; Department of Microbiology, Immunology and Transplantation, KU Leuven, Belgium; Department of Laboratory Medicine, University Hospitals Leuven, Belgium.
  • Van den Wijngaert N; Imec Solutions Department, Imec, Leuven, Belgium. Electronic address: nik.vandenwijngaert@imec.be.
  • Peumans P; Life Science Technologies Department, Imec, Leuven, Belgium.
Biosens Bioelectron ; 217: 114663, 2022 Dec 01.
Article in English | MEDLINE | ID: covidwho-2235885
ABSTRACT
The SARS-CoV-2 pandemic has highlighted the need for improved technologies to help control the spread of contagious pathogens. While rapid point-of-need testing plays a key role in strategies to rapidly identify and isolate infectious patients, current test approaches have significant shortcomings related to assay limitations and sample type. Direct quantification of viral shedding in exhaled particles may offer a better rapid testing approach, since SARS-CoV-2 is believed to spread mainly by aerosols. It assesses contagiousness directly, the sample is easy and comfortable to obtain, sampling can be standardized, and the limited sample volume lends itself to a fast and sensitive analysis. In view of these benefits, we developed and tested an approach where exhaled particles are efficiently sampled using inertial impaction in a micromachined silicon chip, followed by an RT-qPCR molecular assay to detect SARS-CoV-2 shedding. Our portable, silicon impactor allowed for the efficient capture (>85%) of respiratory particles down to 300 nm without the need for additional equipment. We demonstrate using both conventional off-chip and in-situ PCR directly on the silicon chip that sampling subjects' breath in less than a minute yields sufficient viral RNA to detect infections as early as standard sampling methods. A longitudinal study revealed clear differences in the temporal dynamics of viral load for nasopharyngeal swab, saliva, breath, and antigen tests. Overall, after an infection, the breath-based test remains positive during the first week but is the first to consistently report a negative result, putatively signalling the end of contagiousness and further emphasizing the potential of this tool to help manage the spread of airborne respiratory infections.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: J.bios.2022.114663

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: J.bios.2022.114663