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The Rv3874-Rv3875 chimeric protein shows a promiscuous serodiagnostic potential for tuberculosis.
Mahmood, Nasir; Akhter, Mohsina; Hussain, Naveed; Shad, Mohsin; Nisa, Zaib Un; Khan, Imran H; Akhtar, Muhammad Waheed.
  • Mahmood N; School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
  • Akhter M; School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
  • Hussain N; School of Biological Sciences, University of the Punjab, Lahore, Pakistan; Nuffield Department of Medicine, University of Oxford, OX37BN, United Kingdom; School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan.
  • Shad M; School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
  • Nisa ZU; Department of Microbiology, Gulab Devi Chest Hospital, Lahore, Pakistan.
  • Khan IH; Department of Pathology and Laboratory Medicine, University of California, Davis, CA, USA.
  • Akhtar MW; School of Biological Sciences, University of the Punjab, Lahore, Pakistan. Electronic address: mwa.sbs@pu.edu.pk.
Tuberculosis (Edinb) ; 136: 102253, 2022 09.
Article in English | MEDLINE | ID: covidwho-2004564
ABSTRACT
Tuberculosis (TB) stays a major cause of death globally after COVID-19 and HIV. An early diagnosis to control TB effectively, needs a fast reliable diagnostic method with high sensitivity. Serodiagnosis involving polyclonal antibodies detection against an antigen of Mycobacterium tuberculosis (Mtb) in serum samples can be instrumental. In our study, Rv3874 and Rv3875 antigens were cloned, expressed, and purified individually and as a chimeric construct in Escherichia coli BL21. Enzyme-Linked Immunosorbent Assay (ELISA) based findings revealed that the Rv3874-Rv3875 chimeric construct was two-fold more sensitive (59.7%) than the individual sensitivities of Rv3874 (28.4%) and Rv3875 (24.9%) for 201 serum TB positive samples. Furthermore, the fusion construct was a little more sensitive (60.4%) for male subjects than that for females (58.8%). Lastly, our preliminary findings, molecular insights of secondary structure, and statistical and in silico analysis of each construct also advocate that CEP can be considered a better immunodiagnostic tool in addition to previously reported EC skin test.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Tuberculosis / COVID-19 / Mycobacterium tuberculosis Type of study: Diagnostic study Limits: Female / Humans / Male Language: English Journal: Tuberculosis (Edinb) Year: 2022 Document Type: Article Affiliation country: J.tube.2022.102253

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Tuberculosis / COVID-19 / Mycobacterium tuberculosis Type of study: Diagnostic study Limits: Female / Humans / Male Language: English Journal: Tuberculosis (Edinb) Year: 2022 Document Type: Article Affiliation country: J.tube.2022.102253