SARS-CoV-2 CD8+ T cell killing assays using replicating viruses and transgenic antigens.
STAR Protoc
; 3(4): 101699, 2022 Aug 26.
Article
in English
| MEDLINE | ID: covidwho-2004616
ABSTRACT
The quality of an antigen-specific CD8+ T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8+ T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For complete details on the use and execution of this protocol, please refer to Moosmann et al. (2022) and Wagner et al. (2022).
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International databases
Database:
MEDLINE
Language:
English
Journal:
STAR Protoc
Year:
2022
Document Type:
Article
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