Isolation and Molecular Detection of Feline Infectious Peritonitis Virus.
Arch Razi Inst
; 77(5): 1709-1714, 2022 10.
Article
in English
| MEDLINE | ID: covidwho-2006668
ABSTRACT
Feline coronavirus (FCoV) is an enveloped single-stranded RNA virus, affecting wild and domestic cats. Feline infectious peritonitis viruses (FIPV) variants of FCoV cause fatal peritonitis affecting approximately 5% of FCoV infected animals. The present study aimed to detect and isolate the feline infectious peritonitis virus for the first time in Iraq. In this study, 50 samples (fecal swab and peritoneal fluid) were collected from suspected pet cats from different areas of Baghdad, Iraq. The very suitable age was under two years old. Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) was used to detect Feline infectious peritonitis in infected collected samples by the amplification of spike protein (S). The result of real-time RT-PCR revealed that out of 50 samples from suspected cats, 10 samples were positive for FIPV. Moreover, 10 positive samples by real-time RT-PCR were used for the isolation of the virus in chicken embryo fibroblast cell culture. Subsequently, the isolated virus was detected by real-time RT-PCR and then by conventional RT-PCR, followed by electrophoresis.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Cat Diseases
/
Feline Infectious Peritonitis
/
Coronavirus, Feline
Type of study:
Diagnostic study
Topics:
Variants
Limits:
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
Arch Razi Inst
Year:
2022
Document Type:
Article
Affiliation country:
ARI.2022.357997.2135
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