DURABILITY of the HUMORAL RESPONSE to M-RNA BASED ANTI-SARS-COV-2 VACCINES in PATIENTS with AUTOIMMUNE RHEUMATIC DISEASE, A COMPARATIVE STUDY
Annals of the Rheumatic Diseases
; 81:961, 2022.
Article
in English
| EMBASE | ID: covidwho-2009057
ABSTRACT
Background:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral load and its impact on disease outcome in patients with autoimmune rheumatic disease (ARD) are lacking. Also, whether patients with ARD receiving immunomodulators have different viral loads compared to the general population is unknown.Objectives:
To compare the viral load of SARS-CoV-2 and its trending between patients without and with ARD.Methods:
Retrospectively, patients with ARD infected with SARS-CoV-2 were matched by age and sex at a ratio of 12 to patients without ARD and not receiving immunosuppression or immunomodulator drugs. Viral load was determined by the cycle threshold (CT) value measured by a number of platforms (a) Automated Platforms-the Roche Cobas 6800 system using the Cobas SARS-CoV-2 Test targeting the E and orf1a/b genes (Roche, Switzerland) and the Xpert Xpress SARS-CoV-2 targeting the E and N genes (Cepheid, USA);(b) Manual platforms-EZ1 (QIAGEN, USA), QIAsymphony (QIAGEN, USA), and Bioneer ExiPrepTM 96 Virus DNA/RNA kits Catalogue No K4614 (Bioneer, South Korea) extraction with thermal cycling using TaqPath™ PCR COVID-19 Combo Kit targeting the N, S and orf1a/b genes (Thermo Fisher Scientific, USA) on ABI 7500 thermal cyclers. Independent samples t-test was used to compare the mean CT values of the study groups at baseline and at 5 subsequent intervals (1-5.9, 6-11.9, 12-17.9, 18-23.9 and 24-30 days).Results:
Mean age (SD) of 197 cases and 420 controls were 45.2 (11.8) and 44.1 (12.3) years, respectively. Females were predominant in both groups 60% vs. 52%, P=0.053. The most common ARD was rheumatoid arthritis in 82 cases (41.6%), followed by spondyloarthropathy in 33 (16.8%) and systemic lupus ery-thematosus in 31 (15.7%). Of the cases, 67% were on conventional synthetic disease modifying anti-rheumatic drugs (DMARDs), 15.2% on biological DMARDs and 4.6% patients were on rituximab. The mean CT values was signifcantly lower in the ARD group at baseline and persisted till day 24.Conclusion:
Compared to patients without ARD, the viral load of SARS-CoV-2 in patients with ARD is signifcantly higher at baseline testing and persists till day 24. This fnding may indicate that patients with ARD are at higher risk of severe SARS-CoV-2 infection and prolonged potential transmission. Clinical outcome correlation is needed.
disease modifying antirheumatic drug; immunomodulating agent; messenger RNA; rituximab; SARS-CoV-2 vaccine; virus DNA; virus RNA; adult; clinical outcome; comparative study; conference abstract; controlled study; coronavirus disease 2019; cycle threshold value; extraction; female; human; humoral immunity; immunoassay analyzer; immunosuppressive treatment; major clinical study; male; nonhuman; nucleic acid purification robot; outcome assessment; polymerase chain reaction system; rapid test; retrospective study; rheumatic disease; rheumatoid arthritis; SARS coronavirus 2 nucleic acid test kit; Severe acute respiratory syndrome coronavirus 2; South Korea; spondyloarthropathy; Switzerland; systemic lupus erythematosus; virus load
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Topics:
Vaccines
Language:
English
Journal:
Annals of the Rheumatic Diseases
Year:
2022
Document Type:
Article
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