Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.
Vet Res
; 53(1): 67, 2022 Sep 02.
Article
in English
| MEDLINE | ID: covidwho-2009459
ABSTRACT
Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 103 TCID50/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Camelids, New World
/
Coronavirus Infections
/
Middle East Respiratory Syndrome Coronavirus
Type of study:
Experimental Studies
Limits:
Animals
Language:
English
Journal:
Vet Res
Journal subject:
Veterinary Medicine
Year:
2022
Document Type:
Article
Affiliation country:
S13567-022-01084-3
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