Protein E-Peptide Driven Vaccine for Novel Coronavirus: Immunoinformatics
Journal of Kerman University of Medical Sciences
; 29(4):368-377, 2022.
Article
in English
| EMBASE | ID: covidwho-2010569
ABSTRACT
Background:
The COVID-19 pandemic is a red alarm for global health, so researchers around the world are working on it to design an effective vaccine against it. Protein is one of the candidates for vaccine development which plays an important role in virus pathogenesis. Accordingly, this study was done to evaluate the critical characteristic of this protein as a vaccine candidate using in-silico analysis.Methods:
The sequence of SARS-CoV-2-associated E protein was recruited from NCBI and subjected to the IEDB software to evaluate the most potent epitopes. The capacity of the interactions of HLA-I and HLA-II molecules with selective peptides was studied using IEBD tool kit. The E protein sequence was subjected to B cell and T cell tests to realize the most promising peptides that could act as COVID-19 vaccine.Results:
Among the tested peptides for the T cell-test, this study found two interesting epitopes VSEETGTLI and LTALRLCAY that exhibit high binding affinity as a strong indicator to HLA-I and HLA-II alleles together. The results of the analysis demonstrated that some epitopes in the E protein have a relatively higher immunogenicity score based on interaction with HLA-II, such as SEETGTLIVNSVLLF, TLIVNSVLLFLAFVV, LAFVVFLLVTLAILT, LAILTALRLCAYCCN, and SVLLFLAFVVFLLVT. Furthermore, two sequences (FVSEET and PSFYVYSRVKNLNSSRVP) were reported as the selective linear epitopes for B cell, on the surface of SARS-CoV-2 E protein and being Immunogenic.Conclusion:
Since E protein can stimulate favorable immune responses, T and B-cell responses, its evaluation in patients with COVID-19 is of a great importance.
adult; allele; amino acid sequence; article; B lymphocyte; binding affinity; bioinformatics; clinical evaluation; computer model; controlled study; coronavirus disease 2019; human; human cell; immune response; immunogenicity; immunoinformatics; nonhuman; Severe acute respiratory syndrome coronavirus 2; software; T lymphocyte; endogenous compound; epitope; SARS-CoV-2 vaccine
Full text:
Available
Collection:
Databases of international organizations
Database:
EMBASE
Topics:
Vaccines
Language:
English
Journal:
Journal of Kerman University of Medical Sciences
Year:
2022
Document Type:
Article
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