ELECTROCHEMICAL CAPILLARY-FLOW DRIVEN IMMUNOASSAY FOR DETECTION OF SARS-COV-2 NUCLEOCAPSID PROTEIN
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
; : 39-40, 2021.
Article
in English
| Scopus | ID: covidwho-2011121
ABSTRACT
As the COVID-19 pandemic continues, there remains a need for fast, accurate and low-cost diagnostic tests to prevent outbreaks. We have developed an electrochemical capillary-flow driven immunoassay (eCaDI) capable of detecting SARS-CoV-2 nucleocapsid (N) protein in self-administered nasal samples at the point of care (POC). The low-cost device is made of polyester and adhesive films and provides sequential delivery of sample and reagents to a detection zone integrating a screen-printed carbon electrode (SPCE) modified with anti-N protein antibodies from a single addition of sample, automating the steps of an ELISA. The modified electrodes are highly sensitive and selective for COVID-19 N protein and were successfully applied to test clinical samples. The novelty of this work resides in the integration of sensitive electrochemical detection with pump-free capillary-flow assay, providing accuracy at the POC. Previously reported systems are slow and/or require multiple user steps reducing the utility for POC applications relative to the system reported here. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
Capillary-Flow Assay; Electrochemistry; Immunoassay; POC diagnostics; SARS-CoV-2; Adhesives; Capillarity; Capillary electrophoresis; Capillary tubes; Carbon films; Chemical detection; Costs; Electrodes; Immunology; Capillary flow assays; Diagnostic tests; Electrochemicals; Low-cost devices; Low-costs; Nucleocapsid proteins; Nucleocapsids; Point of care; Point of care diagnostic; Coronavirus
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Databases of international organizations
Database:
Scopus
Language:
English
Journal:
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Year:
2021
Document Type:
Article
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