SAMPLE-TO-ANSWER WHOLE BLOOD SEROLOGICAL TEST FOR SARS-COV-2 USING THERMALLY RESPONSIVE VALVES

ABSTRACT

To maximize access to SARS-CoV-2 serological (antibody) tests, point-of-care (PoC) options are necessary. PoC tests require sample-to-answer functionality, which is challenging with whole blood. Here we demonstrate a sample-to-answer SARS-CoV-2 antibody test from whole blood using automated thermally actuated valves. Higher-order alkanes serve as partitions between immunoassay zones (sample/bind, rinse, detection);upon warming, the partitions are liquified, enabling magnetic beads to be pulled through each zone while continuing to partition the reagents. We demonstrate a detection limit of 0.7 ng/mL SARS-CoV-2 antibodies, multiple orders of magnitude lower than clinically relevant concentrations. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.