Rapid SARS-CoV-2 testing with duplexed recombinase polymerase amplification and a bacteriophage internal control
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
; : 855-856, 2021.
Article
in English
| Scopus | ID: covidwho-2011960
ABSTRACT
Without global mass vaccination, COVID-19 will continue to infect and cause serious illness, disproportionately in low- and middle-income countries. Point-of-care and home-based nucleic acid amplification tests (NAATs) are valuable tools to control COVID-19 transmission. Here we present a rapid isothermal NAAT for duplexed detection of SARS-CoV-2 and an MS2 bacteriophage internal control. This assay amplifies RNA in less than 15 minutes, utilizes a low temperature of 39°C, and has fluorescence or visual lateral flow readout. This positions our assay for use in low-cost paper-based nucleic acid diagnostic devices for ultrasensitive and reliable COVID-19 detection in POC or home-based settings. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
Nucleic Acid Testing; Pandemic Management; Paper-Based Device; Point-of-Care Diagnostics; Rapid Virus Testing; Testing for COVID-19; Bacteriophages; Biomolecules; Finance; Temperature; Home-based; Internal controls; Nucleic acids amplification; Paper based devices; Point of care diagnostic; Recombinases; COVID-19
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Collection:
Databases of international organizations
Database:
Scopus
Language:
English
Journal:
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Year:
2021
Document Type:
Article
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