A NOVEL STRATEGY FOR POWER-FREE READOUT OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION USING POLYDOPAMINE INTEGRATED INTO A PAPER DEVICE FOR PATHOGEN DETECTION

ABSTRACT

The result readouts of loop-mediated isothermal amplification (LAMP) still remain challenging because current techniques require bulky equipment and could not give clear visualization. In this study, we developed a paper device to integrate LAMP and a novel strategy for power-free and naked-eye readout of result relied on polydopamine aggregation. The introduced paper device was used to detect DNA extracted from Escherichia coli O157H7 (E. coli O157H7), Enterococcus faecium (E. faecium), and SARS-CoV-2 plasmid. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.