Sequence-Specific Recognition of SARS COV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN)

ABSTRACT

The current outbreak of the SARS-CoV-2 caused the COVID-19 disease to spread rapidly globally. Specific and sensitive detection of SARS-CoV-2 is needed to prevent the disease from spread. Here, we present a solid-state CRISPR-Cas12a-assisted nanopores (SCAN) system to detect SARS-CoV-2. We introduced a new scheme using current drops and dwell times of ssDNA reporter translocation events to estimate the cleavage activity. We validated this scheme by a statistical model approximating the reporter length distribution over the cleavage reaction. We believe that the SCAN would provide a sensitive and specific detection method for SARS-COV-2. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.