SINGLE-STEP QUANTIFICATION OF SPECIFIC NUCLEIC ACID SEQUENCES IN MICROFLUIDICS USING A MULTILABELED HYBRID DNA DUPLEX

ABSTRACT

The rapid and specific detection of nucleic acid sequences is highly demanded for several applications including pathogen diagnostics, quality control of biopharmaceutical products and forensics. Nucleic acid amplification methods based on mixtures of primers and polymerase enzymes such as polymerase chain reaction (PCR) and other isothermal methods are typically the standard approach. Here, using SARS-CoV-2 ORF1ab sequence as a model, we report the development of a simple enzyme-free and single-step competitive hybridization method allowing the specific detection of any type of nucleic acid sequence (ss/dsDNA or RNA) within 15 min with 89% sequence homology and sensitivity in the pM-range. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.