CRISPR-based electrochemical sensor for COVID-19 diagnostics
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
; : 1441-1442, 2021.
Article
in English
| Scopus | ID: covidwho-2012360
ABSTRACT
We report the development of an electrochemical sensor platform for ultrasensitive and rapid detection of SARS-CoV-2 viral RNA that integrates loop-mediated isothermal amplification (LAMP), CRISPR-based detection, and anti-fouling nanocomposite coating. By integrating LAMP amplification with CRISPR, we achieved ultrasensitive detection of SARS-CoV-2 RNA at levels as low as 5 copies µL-1. Data from this electrochemical diagnostic platform was comparable to traditional RT-PCR methodology in a fraction of the time, at low cost, and without requiring laboratory space. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
Biosensor; Cas12a; COVID-19; CRISPR; Electrochemical; RT-LAMP; SARS-CoV-2; Electrochemical sensors; Polymerase chain reaction; RNA; Electrochemicals; Loop mediated isothermal amplifications; Loop-mediated isothermal amplifications; Rapid detection; RT-loop-mediated isothermal amplification; Sensor platform; Ultrasensitive detection; Viral RNA; Coronavirus
Search on Google
Collection:
Databases of international organizations
Database:
Scopus
Language:
English
Journal:
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Year:
2021
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS