SURFACE TENSION-MEDIATED PROCESSING OF WASTEWATER SAMPLES FOR SARS-CoV-2

Strike, W.; Amirsoleimani, A.; Olaleye, A.; Noble, A.; Lewis, K.; Faulkner, L.; Backus, S.; Lindeman, S.; Eterovich, K.; Fraley, M.; Zeitlow, E.; Brann, T.; Hobbs, S.; Monroe, J.; Hibbard, D.; Keck, J.; Berry, S.

Strike, W.; Amirsoleimani, A.; Olaleye, A.; Noble, A.; Lewis, K.; Faulkner, L.; Backus, S.; Lindeman, S.; Eterovich, K.; Fraley, M.; Zeitlow, E.; Brann, T.; Hobbs, S.; Monroe, J.; Hibbard, D.; Keck, J.; Berry, S..

25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; : 849-850, 2021.

Article in English | Scopus | ID: covidwho-2012644

ABSTRACT

ABSTRACT

Wastewater testing for SARS-CoV-2 has emerged as a promising tool for disease surveillance in aggregate populations. We present a novel method to rapidly extract, concentrate, and amplify viral RNA from wastewater using Exclusion-based Sample Preparation (ESP) and RT-PCR. This technology identified potential outbreaks of SARS-CoV-2 at University of Kentucky dormitories, resulting in targeted clinical testing and quarantine procedures. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Keywords

Molecular Diagnostics; SARS-CoV-2; Surface Tension; Diagnosis; Polymerase chain reaction; Clinical testing; Disease surveillance; Novel methods; Sample preparation; University of Kentucky; Viral RNA; Wastewater samples; Coronavirus

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Collection: Databases of international organizations Database: Scopus Language: English Journal: 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 Year: 2021 Document Type: Article

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