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Missense mutations in spike protein of SARS-CoV-2 delta variant contribute to the alteration in viral structure and interaction with hACE2 receptor.
Mahmood, Tousif Bin; Hossan, Mohammad Imran; Mahmud, Shafi; Shimu, Mst Sharmin Sultana; Alam, Md Jahidul; Bhuyan, Md Mahfuzur Rahman; Emran, Talha Bin.
  • Mahmood TB; Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, Bangladesh.
  • Hossan MI; Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, Bangladesh.
  • Mahmud S; Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh.
  • Shimu MSS; Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh.
  • Alam MJ; Department of Applied Chemistry and Chemical Engineering, Noakhali Science and Technology University, Noakhali, Bangladesh.
  • Bhuyan MMR; Department of Biochemistry and Molecular Biology, Noakhali Science and Technology University, Noakhali, Bangladesh.
  • Emran TB; Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, Bangladesh.
Immun Inflamm Dis ; 10(9): e683, 2022 09.
Article in English | MEDLINE | ID: covidwho-2013528
ABSTRACT

INTRODUCTION:

Many of the global pandemics threaten human existence over the decades among which coronavirus disease (COVID-19) is the newest exposure circulating worldwide. The RNA encoded severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus is referred as the pivotal agent of this deadly disease that induces respiratory tract infection by interacting host ACE2 receptor with its spike glycoprotein. Rapidly evolving nature of this virus modified into new variants helps in perpetrating immune escape and protection against host defense mechanism. Consequently, a new isolate, delta variant originated from India is spreading perilously at a higher infection rate.

METHODS:

In this study, we focused to understand the conformational and functional significance of the missense mutations found in the spike glycoprotein of SARS-CoV-2 delta variant performing different computational analysis.

RESULTS:

From physiochemical analysis, we found that the acidic isoelectric point of the virus elevated to basic pH level due to the mutations. The targeted mutations were also found to change the interactive bonding pattern and conformational stability analyzed by the molecular dynamic's simulation. The molecular docking study also revealed that L452R and T478K mutations found in the RBD domain of delta variant spike protein contributed to alter interaction with the host ACE2 receptor.

CONCLUSIONS:

Overall, this study provided insightful evidence to understand the morphological and attributive impact of the mutations on SARS-CoV-2 delta variant.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Angiotensin-Converting Enzyme 2 / SARS-CoV-2 / COVID-19 Topics: Variants Limits: Humans Language: English Journal: Immun Inflamm Dis Year: 2022 Document Type: Article Affiliation country: Iid3.683

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Angiotensin-Converting Enzyme 2 / SARS-CoV-2 / COVID-19 Topics: Variants Limits: Humans Language: English Journal: Immun Inflamm Dis Year: 2022 Document Type: Article Affiliation country: Iid3.683