Printed Capacitive Immunoassay for Detecting SARS-CoV-2 Viral Particles
IEEE Sensors Journal
; : 1-1, 2022.
Article
in English
| Scopus | ID: covidwho-2018960
ABSTRACT
The key to fight against a global pandemic such as COVID-19 is to have low-cost, reliable and fast response diagnostic tools. Electronic biosensors are preferred because of their ease of integration into current centralized health care networks and integration with modern point-of-care testing (POCT) devices. Printed electronic sensors provide a sensitive and reliable diagnostic platform to aid in controlling transmissible diseases. In this work, we demonstrate a fully printed capacitive biosensor. The sensor uses coplanar electrodes, coupled with capture antibodies immobilized on microporous Polyvinylidene-fluoride (PVDF) film to detect the SARS-CoV-2 spike protein in spiked buffer solutions. Antibody immobilization on PVDF surface is confirmed with confocal fluorescent imaging microscopy. Gold nanoparticle (GNP) tagged detection antibodies are also introduced to provide increased sensitivity. The gold nanoparticles provide a reflectance layer which leads to increased capacitance. This increased capacitance can be measured directly and has demonstrated the ability to screen for spiked samples with statistical significance. This fully printed capacitive immunoassay has the potential to be used as a transmissible disease screening and vaccine efficacy assessment tool for resource-limited areas. IEEE
Antibody immobilization; Antibody-antigen binding; Capacitance; Capacitive sensor; Electrodes; Fluorescent imaging; Gold; Imaging; Immune system; Printed electronics; Printed immunoassay; Proteins; PVDF; Sensors; Antibodies; Antigens; Biosensors; Capacitive sensors; Chemical detection; Diagnosis; Fiber optic sensors; Fluorescence imaging; Fluorine compounds; Gold nanoparticles; Immunology; Integration testing; Metal nanoparticles; Antibody-antigen; Antigen binding; Gold nanoparticle; Polyvinylidene fluorides
Full text:
Available
Collection:
Databases of international organizations
Database:
Scopus
Language:
English
Journal:
IEEE Sensors Journal
Year:
2022
Document Type:
Article
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